Probiotic Properties of Lactic Acid Bacteria Isolated from Water-Buffalo Mozzarella Cheese

被引:47
作者
Jeronymo-Ceneviva, Ana Beatriz [1 ]
de Paula, Aline Teodoro [1 ]
Silva, Luana Faria [1 ]
Todorov, Svetoslav Dimitrov [2 ]
Mello Franco, Bernadette Dora G. [2 ]
Penna, Ana Lucia B. [1 ]
机构
[1] UNESP Sao Paulo State Univ, Dept Food Engn & Technol, BR-15054000 Jardim Nazareth, Sao Jose Do Rio, Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food Sci & Expt Nutr, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Probiotic; Lactobacillus; Leuconostoc; Functional food; Dairy; LACTOBACILLUS-PLANTARUM STRAINS; BIOGENIC-AMINE PRODUCTION; IN-VITRO; ANTIBIOTIC-RESISTANCE; GASTROINTESTINAL-TRACT; MULTIPLEX-PCR; MILK; ACIDOPHILUS; ENTEROCOCCI; SAFETY;
D O I
10.1007/s12602-014-9166-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study evaluated the probiotic properties ( stability at different pH values and bile salt concentration, auto-aggregation and co-aggregation, survival in the presence of antibiotics and commercial drugs, study of beta-galactosidase production, evaluation of the presence of genes encoding MapA and Mub adhesion proteins and EF-Tu elongation factor, and the presence of genes encoding virulence factor) of four LAB strains (Lactobacillus casei SJRP35, Leuconostoc citreum SJRP44, Lactobacillus delbrueckii subsp. bulgaricus SJRP57 and Leuconostoc mesenteroides subsp. mesenteroides SJRP58) which produced antimicrobial substances (antimicrobial peptides). The strains survived the simulated GIT modeled in MRS broth, whole and skim milk. In addition, auto-aggregation and the cell surface hydrophobicity of all strains were high, and various degrees of co-aggregation were observed with indicator strains. All strains presented low resistance to several antibiotics and survived in the presence of commercial drugs. Only the strain SJRP44 did not produce the beta-galactosidase enzyme. Moreover, the strain SJRP57 did not show the presence of any genes encoding virulence factors; however, the strain SJRP35 presented vancomycin resistance and adhesion of collagen genes, the strain SJRP44 harbored the ornithine decarboxylase gene and the strain SJRP58 generated positive results for aggregation substance and histidine decarboxylase genes. In conclusion, the strain SJRP57 was considered the best candidate as probiotic cultures for further in vivo studies and functional food products development.
引用
收藏
页码:141 / 156
页数:16
相关论文
共 66 条
  • [51] In vitro adherence and other properties of lactobacilli used in probiotic yoghurt-like products
    Schillinger, U
    Guigas, C
    Holzapfel, WH
    [J]. INTERNATIONAL DAIRY JOURNAL, 2005, 15 (12) : 1289 - 1297
  • [52] Silva LF, 2010, THESIS SAO PAULO STA
  • [53] Biogenic amines in fermented foods
    Spano, G.
    Russo, P.
    Lonvaud-Funel, A.
    Lucas, P.
    Alexandre, H.
    Grandvalet, C.
    Coton, E.
    Coton, M.
    Barnavon, L.
    Bach, B.
    Rattray, F.
    Bunte, A.
    Magni, C.
    Ladero, V.
    Alvarez, M.
    Fernandez, M.
    Lopez, P.
    de Palencia, P. F.
    Corbi, A.
    Trip, H.
    Lolkema, J. S.
    [J]. EUROPEAN JOURNAL OF CLINICAL NUTRITION, 2010, 64 : S95 - S100
  • [54] Screening of lactic acid bacteria toward their selection as a source of chicken probiotic
    Taheri, H. R.
    Moravej, H.
    Tabandeh, F.
    Zaghari, M.
    Shivazad, M.
    [J]. POULTRY SCIENCE, 2009, 88 (08) : 1586 - 1593
  • [55] Boza, a natural source of probiotic lactic acid bacteria
    Todorov, S. D.
    Botes, M.
    Guigas, C.
    Schillinger, U.
    Wiid, I.
    Wachsman, M. B.
    Holzapfel, W. H.
    Dicks, L. M. T.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2008, 104 (02) : 465 - 477
  • [56] Probiotic properties of Lactococcus lactis ssp lactis HV219, isolated from human vaginal secretions
    Todorov, S. D.
    Botes, M.
    Danova, S. T.
    Dicks, L. M. T.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2007, 103 (03) : 629 - 639
  • [57] Potential beneficial properties of bacteriocin-producing lactic acid bacteria isolated from smoked salmon
    Todorov, S. D.
    Furtado, D. N.
    Saad, S. M. I.
    Tome, E.
    Franco, B. D. G. M.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2011, 110 (04) : 971 - 986
  • [58] Evaluation of the probiotic potential and effect of encapsulation on survival for Lactobacillus plantarum ST16Pa isolated from papaya
    Todorov, Svetoslav D.
    Guy LeBlanc, Jean
    Franco, Bernadette D. G. M.
    [J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2012, 28 (03) : 973 - 984
  • [59] Bacteriocinogenic Lactobacillus plantarum ST16Pa isolated from papaya (Carica papaya) - From isolation to application: Characterization of a bacteriocin
    Todorov, Svetoslav D.
    Prevost, Herve
    Lebois, Martine
    Dousset, Xavier
    Guy LeBlanc, Jean
    Franco, Bernadette D. G. M.
    [J]. FOOD RESEARCH INTERNATIONAL, 2011, 44 (05) : 1351 - 1363
  • [60] BACTERIOCINS FROM LACTOBACILLUS PLANTARUM - PRODUCTION, GENETIC ORGANIZATION AND MODE OF ACTION
    Todorov, Svetoslav D.
    [J]. BRAZILIAN JOURNAL OF MICROBIOLOGY, 2009, 40 (02) : 209 - 221