Red-Light-Induced Inhibition of DNA Replication and Amplification by PCR with an Os/Rh Supramolecule

被引：20
作者：
Wang, Jing [1 ]
Newman, Jerry, Jr. [1 ]
Higgins, Samantha L. H. [1 ]
Brewer, Kaitlyn M. [1 ]
Winkel, Brenda S. J. [2 ]
Brewer, Karen J. [1 ]
机构：
[1] Virginia Tech, Dept Chem, Blacksburg, VA 24061 USA
[2] Virginia Tech, Dept Biol Sci, Blacksburg, VA 24061 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 04期
关键词：
DNA amplification; metal complexes; photochemistry; photodynamic therapy; polymerase chain reaction; PHOTODYNAMIC THERAPY; POLYPYRIDYLRUTHENIUM(II) COMPLEXES; IN-VITRO; PHOTOCLEAVAGE; CELLS; CIS-DICHLOROBIS(1,10-PHENANTHROLINE)RHODIUM(III); PHOTOTOXICITY; MECHANISM; CLEAVAGE; RIBOSE;
D O I：
10.1002/anie.201207083
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Bimetallic: Upon irradiation with light in the phototherapeutic window, the bimetallic complex [(bpy)2Os(dpp)RhCl2(phen)] 3+ binds to and cleaves DNA through an oxygen-independent mechanism. PCR is used as a model for in vitro DNA replication. The results show that the metal complex does not interfere with DNA amplification in the dark without O2, but completely inhibits DNA amplification in samples exposed to red light. Taq=polymerase, PS=photosensitizer. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：1262 / 1265
页数：4
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