Detection of Zaire Ebola virus by real-time reverse transcription-polymerase chain reaction, Sierra Leone, 2014

被引：14

作者：

Liu, Licheng ^{[1
]}

Sun, Yang ^{[2
]}

Kargbo, Brima ^{[3
]}

Zhang, Chuntao ^{[4
]}

Feng, Huahua ^{[5
]}

Lu, Huijun ^{[2
]}

Liu, Wenseng ^{[2
]}

Wang, Chengyu ^{[2
]}

Hu, Yi ^{[2
]}

Deng, Yongqiang ^{[2
]}

Jiang, Jiafu ^{[2
]}

Kang, Xiaoping ^{[1
]}

Yang, Honglei ^{[5
]}

Jiang, Yongqiang ^{[1
]}

Yang, Yinhui ^{[1
]}

Kargbo, David ^{[3
]}

Qian, Jun ^{[2
]}

Chen, Weijun ^{[5
,6
]}

机构：

[1] Acad Mil Med Sci, Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

[2] China Mobile Lab Testing Team Sierra Leone, Freetown, Sierra Leone

[3] Minist Hlth & Sanitat, Freetown, Sierra Leone

[4] Natl Inst Food & Drug Control, Beijing 100050, Peoples R China

[5] Beijing BGI GBI Biotech Co Ltd, Beijing 101300, Peoples R China

[6] Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2015年 / 222卷

关键词：

Ebola virus disease; Zaire Ebola virus; Real-time reverse transcription polymerase; chain reaction (rRT-PCR); RAPID DETECTION; HEMORRHAGIC-FEVER; DENGUE VIRUS; PCR; FILOVIRUSES; OUTBREAK; PROVINCE; CHINA;

D O I：

10.1016/j.jviromet.2015.05.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

During the 2014 Ebola virus disease (EVD) outbreak, a real-time quantitative polymerase chain reaction was established to detect and identify the Zaire Ebola virus. We describe the use of this assay to screen 315 clinical samples from EVD suspected person in Sierra Leone. The detection rate in blood samples was 77.81% (207/266), and there were relatively higher detection rate (79.32% and 81.42%, respectively) during the first two weeks after onset of symptoms. In the two weeks that followed, the detection rate declined to 66.67% and 25.00%, respectively. There was the highest virus load at the first week and then decreased. The detection rate in swab samples was 89.79% (44/49). This may be benefit from the included patients. 46 of 49 swab samples were collected from died patients. Taken together, the results presented here indicate that the assay specifically and sensitively detects Zaire Ebola virus. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：62 / 65

页数：4

共 13 条

[1]

[Anonymous], 2014, EB RESP ROADM SIT RE

[2] Real-time PCR for detecting circulating dengue virus in the Guangdong Province of China in 2006 [J].

Bai, Zhijun ;

Liu, Licheng ;

Tu, Zeng ;

Yao, Lisi ;

Liu, Jianwei ;

Xu, Bing ;

Tang, Boheng ;

Liu, Jinhua ;

Wan, Yongji ;

Fang, Meiyu ;

Chen, Weijun .

JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 57 (12) :1547-1552

[3] Emergence of Zaire Ebola Virus Disease in Guinea [J].

Baize, Sylvain ;

Pannetier, Delphine ;

Oestereich, Lisa ;

Rieger, Toni ;

Koivogui, Lamine ;

Magassouba, N'Faly ;

Soropogui, Barre ;

Sow, Mamadou Saliou ;

Keita, Sakoba ;

De Clerck, Hilde ;

Tiffany, Amanda ;

Dominguez, Gemma ;

Loua, Mathieu ;

Traore, Alexis ;

Kolie, Moussa ;

Malano, Emmanuel Roland ;

Heleze, Emmanuel ;

Bocquin, Anne ;

Mely, Stephane ;

Raoul, Herve ;

Caro, Valerie ;

Cadar, Daniel ;

Gabriel, Martin ;

Pahlmann, Meike ;

Tappe, Dennis ;

Schmidt-Chanasit, Jonas ;

Impouma, Benido ;

Diallo, Abdoul Karim ;

Formenty, Pierre ;

Van Herp, Michel ;

Guenther, Stephan .

NEW ENGLAND JOURNAL OF MEDICINE, 2014, 371 (15) :1418-1425

[4] Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, Dengue virus, and Yellow fever virus by real-time reverse transcription-PCR [J].

Drosten, C ;

Göttig, S ;

Schilling, S ;

Asper, M ;

Panning, M ;

Schmitz, H ;

Günther, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (07) :2323-2330

[5]

Fajfr M, 2014, EPIDEMIOL MIKROBI IM, V63, P238

[6] Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak [J].

Gire, Stephen K. ;

Goba, Augustine ;

Andersen, Kristian G. ;

Sealfon, Rachel S. G. ;

Park, Daniel J. ;

Kanneh, Lansana ;

Jalloh, Simbirie ;

Momoh, Mambu ;

Fullah, Mohamed ;

Dudas, Gytis ;

Wohl, Shirlee ;

Moses, Lina M. ;

Yozwiak, Nathan L. ;

Winnicki, Sarah ;

Matranga, Christian B. ;

Malboeuf, Christine M. ;

Qu, James ;

Gladden, Adrianne D. ;

Schaffner, Stephen F. ;

Yang, Xiao ;

Jiang, Pan-Pan ;

Nekoui, Mahan ;

Colubri, Andres ;

Coomber, Moinya Ruth ;

Fonnie, Mbalu ;

Moigboi, Alex ;

Gbakie, Michael ;

Kamara, Fatima K. ;

Tucker, Veronica ;

Konuwa, Edwin ;

Saffa, Sidiki ;

Sellu, Josephine ;

Jalloh, Abdul Azziz ;

Kovoma, Alice ;

Koninga, James ;

Mustapha, Ibrahim ;

Kargbo, Kandeh ;

Foday, Momoh ;

Yillah, Mohamed ;

Kanneh, Franklyn ;

Robert, Willie ;

Massally, James L. B. ;

Chapman, Sinead B. ;

Bochicchio, James ;

Murphy, Cheryl ;

Nusbaum, Chad ;

Young, Sarah ;

Birren, BruceW. ;

Grant, Donald S. ;

Scheiffelin, John S. .

SCIENCE, 2014, 345 (6202) :1369-1372

[7] Detection of a novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome in Henan Province, China, using real-time reverse transcription PCR [J].

Huang, Xueyong ;

Liu, Licheng ;

Du, Yanhua ;

Ma, Hongxia ;

Mu, Yujiao ;

Wang, Pengzhi ;

Ma, Hong ;

Tang, Xiaoyan ;

Wang, Haifeng ;

Kang, Kai ;

Zhang, Shiqiang ;

Wu, Weili ;

Chen, Haomin ;

Liu, Guohua ;

Yang, Yinhui ;

Jian, Yongqiangj ;

Xu, Bianli ;

Chen, Weijun .

JOURNAL OF MEDICAL MICROBIOLOGY, 2013, 62 :1060-1064

[8]

Huang Yi, 2012, Virologica Sinica, V27, P273, DOI 10.1007/s12250-012-3252-y

[9] EVALUATION OF PERCEIVED THREAT DIFFERENCES POSED BY FILOVIRUS VARIANTS [J].

Kuhn, Jens H. ;

Dodd, Lori E. ;

Wahl-Jensen, Victoria ;

Radoshitzky, Sheli R. ;

Bavari, Sina ;

Jahrling, Peter B. .

BIOSECURITY AND BIOTERRORISM-BIODEFENSE STRATEGY PRACTICE AND SCIENCE, 2011, 9 (04) :361-371

[10] Proposal for a revised taxonomy of the family Filoviridae: classification, names of taxa and viruses, and virus abbreviations [J].

Kuhn, Jens H. ;

Becker, Stephan ;

Ebihara, Hideki ;

Geisbert, Thomas W. ;

Johnson, Karl M. ;

Kawaoka, Yoshihiro ;

Lipkin, W. Ian ;

Negredo, Ana I. ;

Netesov, Sergey V. ;

Nichol, Stuart T. ;

Palacios, Gustavo ;

Peters, Clarence J. ;

Tenorio, Antonio ;

Volchkov, Viktor E. ;

Jahrling, Peter B. .

ARCHIVES OF VIROLOGY, 2010, 155 (12) :2083-2103

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