Differential effects of ethanol in the nucleus accumbens shell of alcohol-preferring (P), alcohol-non-preferring (NP) and Wistar rats: A proteomics study

被引：43
作者：
McBride, William J. [1 ,2 ]
Schultz, Jonathan A. [1 ]
Kimpel, Mark W. [1 ]
McClintick, Jeanette N. [3 ]
Wang, Mu [2 ,4 ]
You, Jinsam [4 ]
Rodd, Zachary A. [1 ]
机构：
[1] Indiana Univ, Sch Med, Inst Psychiat Res, Dept Psychiat, Indianapolis, IN 46202 USA
[2] Indiana Univ, Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USA
[3] Indiana Univ, Sch Med, Ctr Med Genom, Indianapolis, IN 46202 USA
[4] Monarch Life Sci, Indianapolis, IN 46202 USA
来源：
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR | 2009年 / 92卷 / 02期
关键词：
Alcohol-preferring rats; Alcohol-non-preferring rats; Nucleus accumbens shell; Protein expression; Proteomics; Alcohol; NUCLEOTIDE EXCHANGE FACTOR; GENE-EXPRESSION; MICROARRAY ANALYSIS; PROTEIN EXPRESSION; SYNAPTIC PLASTICITY; TOLERANCE; DENSITY; LINES; HIPPOCAMPUS; INTERACTS;
D O I：
10.1016/j.pbb.2008.12.019
中图分类号：
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号：
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要：
The objective of this study was to determine the effects of ethanol injections on protein expression in the nucleus accumbens shell (ACB-sh) of alcohol-preferring (P), alcohol-non-preferring (NP) and Wistar (W) rats. Rats were injected for 5 consecutive days with either saline or 1 g/kg ethanol; 24 h after the last injection, rats were killed and brains obtained. Micro-punch samples of the ACB-sh were homogenized; extracted proteins were subjected to trypsin digestion and analyzed with a liquid chromatography-mass spectrometer procedure. Ethanol changed expression levels (1.15-fold or higher) of 128 proteins in NP rats, 22 proteins in R and 28 proteins in W rats. Few of the changes observed with ethanol treatment for NP rats were observed for P and W rats. Many of the changes occurred in calcium-calmodulin signaling systems. G-protein signaling systems, synaptic structure and histones. Approximately half the changes observed in the ACB-sh of P rats were also observed for W rats. Overall, the results indicate a unique response to ethanol of the ACB-sh of NP rats compared to P and W rats; this unique response may reflect changes in neuronal function in the ACB-sh that could contribute to the low alcohol drinking behavior of the NP line. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：304 / 313
页数：10
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