Stabilizing selenium nanoparticles with chymotrypsin: The effect of pH and nanoparticle-enzyme concentration ratios on the stability of nanocomplexes

被引：13

作者：

Borovikova, L. N. ^{[1
]}

Titova, A. V. ^{[1
]}

Matveeva, N. A. ^{[1
]}

Pisarev, O. A. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Macromol Cpds, St Petersburg 199004, Russia

来源：

RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY A | 2013年 / 87卷 / 06期

关键词：

selenium; chymotrypsin; nanoparticles; nanocomplexes; adsorption; protein;

D O I：

10.1134/S0036024413060058

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Conditions of the formation of soluble chymotrypsin complexes with selenium nanoparticles are studied. It is established that the aggregative stability of the nanocomplexes is determined by their conditions of preparation, depending also on the medium pH and ratio of selenium: chymotrypsin concentration. It is shown that chymotrypsin has a "protective" effect due to its hydrophobic adsorption on the surface of selenium nanoparticles and hence the hydrophilization of their surfaces due to ionized groups of the protein.

引用

页码：998 / 1001

页数：4

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