MT1-MMP regulates MMP-2 expression and angiogenesis-related functions in human umbilical vein endothelial cells

被引：19

作者：

Lee, Hsinyu ^{[1
,2
,5
,6
,7
]}

Chang, Kai-Wei ^{[3
]}

Yang, Hsi-Yuan ^{[3
]}

Lin, Po-Wei ^{[2
]}

Chen, Shee-Uan ^{[4
]}

Huang, Yuan-Li ^{[8
]}

机构：

[1] Natl Taiwan Univ, Dept Life Sci, Taipei 10764, Taiwan

[2] Natl Taiwan Univ, Inst Zool, Taipei 10764, Taiwan

[3] Natl Taiwan Univ, Inst Mol & Cellular Biol, Taipei 10764, Taiwan

[4] Natl Taiwan Univ Hosp, Dept Obstet & Gynecol, Taipei 100, Taiwan

[5] Natl Taiwan Univ, Ctr Biotechnol, Taipei 10764, Taiwan

[6] Natl Taiwan Univ, Angiogenesis Res Ctr, Taipei 10764, Taiwan

[7] Natl Taiwan Univ, Res Ctr Dev Biol & Regenerat Med, Taipei 10764, Taiwan

[8] Asia Univ, Coll Hlth Sci, Dept Biotechnol, Taichung, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 437卷 / 02期

关键词：

MT1-MMP; MMP-2; HUVECs; Angiogenesis; MEMBRANE-TYPE-1; MATRIX-METALLOPROTEINASE; 1-MATRIX METALLOPROTEINASE; MYOCARDIAL-INFARCTION; TUMOR ANGIOGENESIS; TARGETED DELETION; TISSUE INHIBITOR; ACTIVATION; GROWTH; MECHANISM; SURFACE;

D O I：

10.1016/j.bbrc.2013.06.046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Membrane type 1 (MT1)-MMP is a member of matrix metalloproteinases (MMPs) that regulates extracellular matrix remodeling. In addition, MT1-MMP also serves as a multi-functional protein. However, the functional role of MT1-MMP in human endothelial cells remains unclear. In this study we use real-time PCR and Western blotting to demonstrate for the first time that MMP-2 expression is regulated by MT1-MMP in human endothelial cells. Moreover, MMP-2 activity is also modulated by MT1-MMP. In addition we found that endothelial cells, ECM adhesion and human endothelial cell tube formation, which are known to be regulated by MMP-2, are blocked by MT1-MMP siRNA. These results suggest that MT1-MMP plays an important role in regulating angiogenesis in human endothelial cells. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.

引用

页码：232 / 238

页数：7

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