25-Hydroxyvitamin D assays: Potential interference from other circulating vitamin D metabolites

被引:44
作者
Carter, G. D. [1 ]
Jones, J. C. [1 ]
Shannon, J. [1 ]
Williams, E. L. [1 ]
Jones, G. [2 ]
Kaufmann, M. [2 ]
Sempos, C. [3 ]
机构
[1] Charing Cross Hosp, Fulham Palace Rd, London W6 8RF, England
[2] Queens Univ, Dept Biomed & Mol Sci, Kingston, ON K7L 3N6, Canada
[3] NIH, Bldg 10, Bethesda, MD 20892 USA
关键词
DEQAS; 25-Hydroxyvitamin D; 3-epi-25-Hydroxyvitamin D; 24,25-Dihydroxyvitamin D; Interference; 24,25-DIHYDROXYVITAMIN D; SERUM; INFANTS;
D O I
10.1016/j.jsbmb.2015.12.018
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The vitamin D External Quality Assessment Scheme (DEQAS) for 25-hydroxyvitamin D (25-OHD) has approximately 1100 participants in 53 countries using 26 different methods or variants of methods (October 2014). In April 2015, the scheme was extended to cover 24,25-dihydroxyvitamin D. (24,25 (OH)(2)D). Since 2013, the 25-OHD scheme has been accuracy-based with values assigned by the NIST reference measurement procedure (RMP). DEQAS is uniquely placed to assess the accuracy (bias) and specificity of 25-OHD methods in a routine laboratory setting. Other vitamin D metabolites are known to interfere in 25-OHD assays and DEQAS has distributed samples spiked with 3-epi-25-OHD3 (52.4 nmol/L), 24R,25(OH)(2)D-3 (14.4 nmol/L) and 245,25(OH)(2)D-3 (57.9 nmol/L). The 3-epimer showed a cross reactivity of 56% in a competitive protein binding assay but was not detected in any antibody-based methods. Not all HPLC/UV or LC-MS/MS methods were able to resolve 3-epi-25-OHD3 from 25-OHD3 and thus overestimated total 25-OHD:The cross reactivity of 24R,25(OH)(2)D-3 (245,25(OH)(2)D-3) ranged from <5% (<5%) to 548% (643%) in ligand binding assays. Both 24-hydroxylated metabolites were resolved by HPLC/UV and LC-MS/MS methods and thus caused no complications in the measurement of 25-OHD. Most antibodies to 25-OHD are known to cross-react with dihydroxylated metabolites but interference in some assays was far greater than expected. This may be related to the anomalous behaviour of exogenously added metabolites in these 25-OHD methods. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:134 / 138
页数:5
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