Protective effect of allicin on high glucose/hypoxia-induced aortic endothelial cells via reduction of oxidative stress

被引:33
作者
Wang, Shu-Li [1 ,2 ]
Liu, De-Shan [1 ]
Liang, Er-Shun [1 ,3 ]
Gao, Yan-Hua [1 ]
Cui, Ying [4 ]
Liu, Yu-Zhao [4 ]
Gao, Wei [1 ]
机构
[1] Shandong Univ, Qilu Hosp, Dept Tradit Chinese Med, Jinan 250012, Shandong, Peoples R China
[2] Linyi Peoples Hosp, Dept Geriatr, Linyi 276003, Shandong, Peoples R China
[3] Shandong Univ, Sch Med, Jinan 250012, Shandong, Peoples R China
[4] Shandong Univ, Qilu Hosp, Key Lab Cardiovasc Remodeling & Funct Res, Jinan 250012, Shandong, Peoples R China
关键词
oxidative stress; allicin; NADPH oxidase 4; hypoxia-inducible factor-1 alpha; 8-hydroxydeoxyguanosine; PROTEIN-KINASE-C; DIABETES-MELLITUS; GENE-EXPRESSION; SUPEROXIDE-PRODUCTION; NAD(P)H OXIDASE; OXYGEN; ACTIVATION; DISEASE; COMPLICATIONS; NEPHROPATHY;
D O I
10.3892/etm.2015.2708
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
This study was designed to explore the protective effect of allicin on aortic endothelial cell injury induced by high glucose/hypoxia and to investigate the corresponding mechanisms. The primary-cultured murine aortic endothelial cells were subcultured. The third passage of cells was adopted and randomly divided into five groups: The normal group (NG), the mannitol group (MG), the high-glucose/hypoxia group (HG), the allicin group (AG) and the protein kinase C (PKC) inhibitor group (GG). The general morphology was observed under an inverted phase-contrast microscope and cell viability was assessed using the MTT assay. Intracellular reactive oxygen species (ROS) levels in the endothelial cells were quantified using dihydroethidium staining. The levels of 8-hydroxydeoxyguanosine (8-OHdG), nuclear factor-kappa B (NF-kappa B), NADPH oxidase 4 (Nox4) and hypoxia-inducible factor-la (HIP-la) and the activity of PKC were measured using ELISA. A quantitative polymerase chain reaction (qPCR) was adopted to evaluate the mRNA expression of Nox4, HIP-la and NF-kappa B. The altered cell morphology observed in HG was notably ameliorated in the AG and GG. The protein levels of 8-OHdG, NF-kappa B, Nox4, HIP-la and PKC in the HG were higher than those in the other groups. Furthermore, the cell viability in the AG was significantly increased and the protein levels of 8-OHdG, NF-kappa B, Nox4, HIP-la and PKC were significantly decreased compared with those in the HG. The ROS production was found to be increased in the HG cells, while there was a significant decrease in the AG cells. These data indicate that allicin exerts a protective effect against high glucose/hypoxia-induced injury in aortic endothelial cells through its antioxidative action, which may involve the inhibition of the PKC pathway and regulation of HIF-1 alpha.
引用
收藏
页码:1394 / 1400
页数:7
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