HPTLC and Spectrophotometric Estimation of Febuxostat and Diclofenac Potassium in Their Combined Tablets

被引:20
作者
El-Yazbi, Fawzi A. [1 ]
Amin, Omayma A. [1 ]
El-Kimary, Eman I. [1 ]
Khamis, Essam F. [1 ]
Younis, Sameh E. [2 ]
机构
[1] Univ Alexandria, Dept Pharmaceut Analyt Chem, Fac Pharm, Alexandria 21521, Egypt
[2] Pharos Univ Alexandria, Fac Pharm & Drug Mfg, Dept Analyt & Pharmaceut Chem, Canal El Mahmoudia St, Alexandria, Egypt
关键词
RP-HPLC METHOD;
D O I
10.1093/chromsci/bmw091
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An accurate, precise, rapid, specific and economic high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous quantitative determination of febuxostat (FEB) and diclofenac potassium (DIC). The chromatographic separation was performed on precoated silica gel 60 GF(254) plates with chloroform-methanol 7:3 (v/v) as the mobile phase. The developed plates were scanned and quantified at 289 nm. Experimental conditions including band size, mobile phase composition and chamber-saturation time were critically studied, and the optimum conditions were selected. A satisfactory resolution (R-s = 2.67) with R-F 0.48 and 0.69 and high sensitivity with limits of detection of 4 and 7 ng/band for FEB and DIC, respectively, were obtained. In addition, derivative ratio and ratio difference spectrophotometric methods were established for the analysis of such a mixture. All methods were validated as per the ICH guidelines. In the HPTLC method, the calibration plots were linear between 0.01-0.55 and 0.02-0.60 mu g/band, for FEB and DIC, respectively. For the spectrophotometric methods, the calibration graphs were linear between 2-14 and 4-18 mu g/mL for FEB and DIC, respectively. The simplicity and specificity of the proposed methods suggest their application in quality control analysis of FEB and DIC in their raw materials and tablets. A comparison of the proposed methods with the existing methods is presented.
引用
收藏
页码:1146 / 1152
页数:7
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