Characterization of a natural Plum pox virus isolate bearing a truncated coat protein

被引：7

作者：

Szathmary, Erzsebet ^{[1
]}

Nadudvari, Julia Novak ^{[2
]}

Szabo, Laszlo ^{[3
]}

Tobias, Istvan ^{[4
]}

Balazs, Ervin ^{[5
]}

Palkovics, Laszlo ^{[1
]}

机构：

[1] Corvinus Univ Budapest, Fac Hort Sci, Dept Plant Pathol, H-1118 Budapest, Hungary

[2] Agr Biotechnol Ctr, H-2100 Godollo, Hungary

[3] Hungarian Acad Sci, Inst Nanochem & Catalysis, Chem Res Ctr, H-1025 Budapest, Hungary

[4] Hungarian Acad Sci, Inst Plant Protect, H-1022 Budapest, Hungary

[5] Hungarian Acad Sci, Agr Res Inst, H-2462 Martonvasar, Hungary

来源：

ARCHIVES OF VIROLOGY | 2009年 / 154卷 / 01期

关键词：

NUCLEOTIDE-SEQUENCE; RNA RECOMBINATION; CAPSID PROTEIN; PLANT-VIRUS; N-TERMINUS; POTYVIRUS; STRAIN; PPV; EVOLUTION; SUBGROUP;

D O I：

10.1007/s00705-008-0281-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plum pox virus (PPV) isolates were collected in Hungary from plum varieties. PCR targeting the 3' genomic region resulted in a shorter PCR product in the case of the B1298 isolate bearing a 135-nucleotide deletion in frame in the N-terminal part of the coat protein (CP). The isolate was aphid-transmissible and the virion diameter was reduced compared to PPV-SK68. Detectability of this isolate by Western blot varied according to the antibody used. Integration of the deleted CP gene into an infectious PPV clone had no effect on infectivity and symptomatology. In competition experiments, B1298 had a considerable advantage in virus accumulation.

引用

页码：141 / 145

页数：5

共 31 条

[1] [Anonymous], 1989, Molecular Cloning: A Laboratory Manual
[2] A nonviral peptide can replace the entire N terminus of zucchini yellow mosaic potyvirus coat protein and permits viral systemic infection
Arazi, T
Shiboleth, YM
Gal-on, A
[J]. JOURNAL OF VIROLOGY, 2001, 75 (14) : 6329 - 6336
[3] AMINO-ACID SUBSTITUTIONS IN THE COAT PROTEIN RESULT IN LOSS OF INSECT TRANSMISSIBILITY OF A PLANT-VIRUS
ATREYA, PL
ATREYA, CD
PIRONE, TP
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) : 7887 - 7891
[4] Candresse T., 1994, Bulletin OEPP, V24, P585, DOI 10.1111/j.1365-2338.1994.tb01072.x
[5] 3'-TERMINAL SEQUENCE OF THE PLUM POX VIRUS PS AND O6 ISOLATES - EVIDENCE FOR RNA RECOMBINATION WITHIN THE POTYVIRUS GROUP
CERVERA, MT
RIECHMANN, JL
MARTIN, MT
GARCIA, JA
[J]. JOURNAL OF GENERAL VIROLOGY, 1993, 74 : 329 - 334
[6] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX
DEVEREUX, J
HAEBERLI, P
SMITHIES, O
[J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (01) : 387 - 395
[7] Dolja V V, 1995, Virology, V206, P1007, DOI 10.1006/viro.1995.1023
[8] DISTINCT FUNCTIONS OF CAPSID PROTEIN IN ASSEMBLY AND MOVEMENT OF TOBACCO ETCH POTYVIRUS IN PLANTS
DOLJA, VV
HALDEMAN, R
ROBERTSON, NL
DOUGHERTY, WG
CARRINGTON, JC
[J]. EMBO JOURNAL, 1994, 13 (06) : 1482 - 1491
[9] Variation and evolution of plant virus populations
García-Arenal, F
Fraile, A
Malpica, JM
[J]. INTERNATIONAL MICROBIOLOGY, 2003, 6 (04) : 225 - 232
[10] Geographically and temporally distant natural recombinant isolates of Plum pox virus (PPV) are genetically very similar and form a unique PPV subgroup
Glasa, M
Palkovics, L
Komínek, P
Kabonne, G
Pittnerová, S
Kúdela, O
Candresse, T
Subr, Z
[J]. JOURNAL OF GENERAL VIROLOGY, 2004, 85 : 2671 - 2681

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