The transient expression of Klf4 and Klf5 during adipogenesis depends on GSK3β activity

被引：22

作者：

Cervantes-Camacho, Carla ^{[1
]}

Beltran-Langarica, Alicia ^{[1
]}

Ochoa-Uribe, Ann-Karoll ^{[1
]}

Marsch-Moreno, Meytha ^{[1
]}

Ayala-Sumuano, Jorge-Tonatiuh ^{[2
]}

Velez-delValle, Cristina ^{[1
]}

Kuri-Harcuch, Walid ^{[1
]}

机构：

[1] Ctr Invest & Estudios Avanzados, Inst Politecn Nacl, Dept Cell Biol, Mexico City, DF, Mexico

[2] Univ Nacl Autonoma Mexico, Inst Neurobiol, Queretaro, Mexico

来源：

ADIPOCYTE | 2015年 / 4卷 / 04期

基金：

芬兰科学院;

关键词：

adipogenesis; Cebpb; differentiation; GSK3; Klf4; Klf5; retinoic acid; SREBP1a; sterol regulatory element binding transcription factor 1a protein; PPAR; Pparg2; peroxisome proliferator activated receptor gamma protein; gene; SREBP1c; sterol regulatory element binding transcription factor 1 protein; C; EBP; Cebpa; CCAAT; enhancer binding protein protein; KLF4; Kruppel-like factor 4 protein; KFL5; Kruppel-like factor 5 protein; MAPK; mitogen-activated protein kinase (MAPK); glycogen synthase kinase 3 protein; St; Staurosporine; Dex; Dexamethasone; Mix; methyl isobutyl xanthine; dexamethasone; St-Dex; Staurosporine-Dexamethasone; N-Ad; non-adipogenic medium; CHX; Cycloheximide; pS-empty; pCMVSport6 empty vector; Ps-Klf4; pCMVSport6Klf4; RA; all-trans retinoic acid; ADIPOSE CONVERSION; ADIPOCYTE DIFFERENTIATION; RETINOIC ACID; 3T3-F442A CELLS; TRANSCRIPTION FACTOR; BINDING-PROTEINS; GENE-EXPRESSION; KEY REGULATOR; C/EBP-BETA; 3T3; CELLS;

D O I：

10.1080/21623945.2015.1007823

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Adipogenesis is regulated by a complex cascade of transcriptional factors, among them KLF4. This factor was previously shown to be necessary for adipose differentiation. We found that GSK3 activity was required for Klf4 and Klf5 expression during adipogenesis. In addition, retinoic acid inhibited Klf4 and Klf5 expression but not that of Cebpb. Protein synthesis inhibition showed that the transient expression of Klf4, Cebpb and Klf5 during early adipogenesis seemed to require a yet unknown protein for their repression. We also found that Klf4 forced expression in 3T3-F442A cells cultured under non-adipogenic conditions did not induce adipogenesis, nor the expression of Cebpb or Klf5, a Cebpb target gene, showing that KLF4 was not sufficient for adipose differentiation to take place. This would suggest that a more complex combination of molecular pathways not yet understood, is involved during early adipogenesis.

引用

页码：248 / 255

页数：8

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