Primers for (1,3)-β-glucan synthase gene amplification and partial characterization of the enzyme in Ganoderma lucidum

被引：3

作者：

Valeria Guerrero-Torres, Jessica ^{[1
]}

Mata, Gerardo ^{[2
]}

Martinez-Carrera, Daniel ^{[3
]}

Garibay-Orijel, Claudio ^{[1
]}

Garibay-Orijel, Roberto ^{[4
]}

机构：

[1] Inst Politecn Nacl, Dept Bioingn, Unidad Profes Interdisciplinaria Biotecnol, Mexico City, DF, Mexico

[2] Inst Ecol AC, Xalapa, Veracruz, Mexico

[3] Colegio Postgrad Ciencias Agr COLPOS, Puebla, Mexico

[4] Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico

来源：

REVISTA IBEROAMERICANA DE MICOLOGIA | 2013年 / 30卷 / 04期

关键词：

(1,3)-beta-glucan synthase; Ganoderma lucidum; Gene; Primers; SACCHAROMYCES-CEREVISIAE; GENOME; IDENTIFICATION; INSIGHTS; HOMOLOG; SUBUNIT; PROTEIN; ENCODES; PCR;

D O I：

10.1016/j.riam.2012.12.006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: beta-(1,3)(1,6)-D-glucan is fungal cell wall component that has demonstrated immunomodulatory and anti-cancer effects. The (1,3)-beta-glucan synthase is one of the main enzymes involved in its biosynthesis. Aims: To design primers to partially amplify and characterize the (1,3)-beta-glucan synthase gene and to determine them in Ganoderma lucidum (G. Lucidum) strain CP-132. Methods: The primers were designed on the basis of homologous genes in other fungi. Then, using the PCR technique, primers were tested using DNA extracted from the G. lucidum strain CP-382. Amplified sequences were compared with those from the GenBank. Results: Three primer pairs were designed; all of them produced amplicons of the expected size. The sequences obtained with primer pairs BGS2113UmF and BGS3097UmR, and BGS547UmF and BGS2113UmR matched with 2 sections of the (1,3)-beta-glucan synthase gene. The deduced amino acid sequences showed high similarity with homologous genes from other fungi, particularly with those of the Agaricomycetes class. Conclusions: The primer design to partially amplify the (1,3)-beta-glucan synthase gene of G. lucidum using sequences from homologous genes was successful. These primers will allow to characterize this important enzyme in a wide group of fungi. (C) 2012 Revista Iberoamericana de Micologia. Published by Elsevier Espana, S.L. All rights reserved.

引用

页码：267 / 270

页数：4

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