Cyclic mechanical strain regulates the development of engineered smooth muscle tissue

被引:349
作者
Kim, BS
Nikolovski, J
Bonadio, J
Mooney, DJ [1 ]
机构
[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Biomed Sci, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA
[4] Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USA
[5] Select Genet, San Diego, CA 92121 USA
关键词
smooth muscle; tissue engineering; elastin; collagen; mechanical stimulation;
D O I
10.1038/13671
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We show that the appropriate combinations of mechanical stimuli and polymeric scaffolds can enhance the mechanical properties of engineered tissues. The mechanical properties of tissues engineered from cells and polymer scaffolds are significantly lower than the native tissues they replace. We hypothesized that application of mechanical stimuli to engineered tissues would alter their mechanical properties. Smooth muscle tissue was engineered on two different polymeric scaffolds and subjected to cyclic mechanical strain. Short-term application of strain increased proliferation of smooth muscle cells (SMCs) and expression of collagen and elastin, but only when SMCs were adherent to specific scaffolds. Long-term application of cyclic strain upregulated elastin and collagen gene expression and led to increased organization in tissues. This resulted in more than an order of magnitude increase in the mechanical properties of the tissues.
引用
收藏
页码:979 / 983
页数:5
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