The T/B cell interaction involved in induction of the mouse IgG2a(b) suppression is restricted by major histocompatibility complex class I, but not class II molecules

To determine the major histocompatibility complex (MHC) restriction of the T/B cell interaction involved in a negative regulation of Ig production, we used mouse model of T cell-induced IgG2a(b) suppression in vivo. Normal or specifically triggered T splenocytes from mice of the Igh(a) haplotype, when neonatally transferred into histocompatible Igh(a/b) heterozygotes, are able to induce a specific and total suppression of the IgG2a(b) allotype. Nevertheless, only transfer of IgG2a(b)-primed Igh(a)T splenocytes induces this suppression in Igh(b/b) homozygous congenic mice in which the whole IgG2a isotype production is inhibited. This suppression is chronically maintained by CD8(+) T cells, but can be experimentally reversed. We have established that the suppression induction required a CD4(+)CD8(+) T cell cooperation and operated via the recognition by the involved TCR of C gamma 2a(b)-derived peptides presented by the target B cells in an MHC haplotype-restricted manner. Here, by using Igh(b) mice genetically deficient for MHC class I (beta 2-microglobulin(%), or beta 2(%)) or class II (I-A beta(%)) molecules, we demonstrate functionally that the suppression induction implicates an MHC class I-, but not class II-restricted interaction. Indeed, the anti-IgG2a(b) T cells transferred into Igh(b) H-2(b) I-A beta(%) mice carry out the suppression process normally, while in Igh(b) H-2(b) beta 2(%) recipients, their suppression induction capacity is significantly inhibited. Moreover, the C gamma 2a(b) 103-118 peptide, identified as the sole C gamma 2a(b)-derived peptide able to amplify the anti-IgG2a(b) T cell reactivity in Igh(a) H-2(b) mice, is also able to stabilize the H-2D(b), but not the H-2K(b) class I molecules at the surface of RMA-S (TAP2(-), H-2(b)) cells. These results indicate that, despite the CD4(+)/CD8(+) T cell cooperation during the induction phase of suppression only MHC class I molecule expression is required at the surface of IgG2a(b+) B cells for suppression establishment.