Generation of mesothelial progenitor-like cells from mouse-induced pluripotent stem cells

被引：3

作者：

Inagaki, Natsuko F. ^{[1
,2
]}

Inagaki, Fuyuki F. ^{[3
,4
]}

Kokudo, Norihiro ^{[3
,4
]}

Miyajima, Atsushi ^{[1
]}

机构：

[1] Univ Tokyo, Inst Quantitat Biosci, Tokyo, Japan

[2] Natl Ctr Global Hlth & Med, Dept Lipid Signaling, Tokyo, Japan

[3] Univ Tokyo, Grad Sch Med, Dept Surg, Hepatobillary Pancreat Surg Div,Artificial Organ, Tokyo, Japan

[4] Natl Ctr Global Hlth & Med, Dept Surg, Tokyo, Japan

来源：

FEBS LETTERS | 2019年 / 593卷 / 04期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

cell sheet; induced pluripotent stem cells; liver regeneration; mesothelial cells; postoperative adhesion; POSTOPERATIVE ADHESION; BIORESORBABLE MEMBRANES; LIVER; HEPATECTOMY; TRANSPLANTATION; TUMORIGENICITY; REGENERATION; OBSTRUCTION; EFFICACY; CANCER;

D O I：

10.1002/1873-3468.13325

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mesothelial cells, which cover the surface of visceral organs and serous cavities in mammals, play a crucial role in preventing adhesion. We previously reported that primary mesothelial progenitor cells (MPCs) can not only prevent postoperative adhesion but also promote liver regeneration after hepatectomy. Induced pluripotent stem cells (iPSCs) have the potential to be used for regenerative medicine. Here, we have established a differentiation protocol for mouse iPSC-derived MPCs (miMPCs) via the exposure to defined factors, as well as purification using MPC-specific cell surface antigens. Furthermore, the miMPCs had the ability to suppress postoperative adhesion and facilitate liver regeneration. This is the first report highlighting the generation of functional miMPCs, which may offer potential for de novo cell therapy.

引用

页码：386 / 394

页数：9

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