Insight into the heat resistance of fish via blood: Effects of heat stress on metabolism, oxidative stress and antioxidant response of olive flounder Paralichthys olivaceus and turbot Scophthalmus maximus

被引：85

作者：

Lu, Yunliang ^{[1
,2
]}

Wu, Zhihao ^{[1
,2
]}

Song, Zongcheng ^{[3
]}

Xiao, Peng ^{[1
,2
]}

Liu, Ying ^{[4
]}

Zhang, Peijun ^{[1
,2
]}

You, Feng ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China

[3] Shenghang Aquat Sci & Technol Co Ltd, Weihai 264200, Peoples R China

[4] Dalian Ocean Univ, Sch Marine Sci & Environm Engn, Dalian 116023, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2016年 / 58卷

基金：

中国国家自然科学基金;

关键词：

Paralichthys olivaceus; Scophthalmus maximus; Heat stress; Heat-resistance; Anaerobic metabolism; Oxidative stress; TROUT ONCORHYNCHUS-MYKISS; OREOCHROMIS-NILOTICUS L; MESSENGER-RNA EXPRESSION; CRAB SCYLLA-SERRATA; WATER TEMPERATURE; SEX-DIFFERENTIATION; GILL MITOCHONDRIA; JAPANESE FLOUNDER; CATALASE ACTIVITY; IMMUNE-RESPONSE;

D O I：

10.1016/j.fsi.2016.09.008

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

High temperature has direct confinement on fish survival and growth, especially under the background of global warming. Selection of fish line with heat resistance is an important means to address this problem. In the present study, we analyzed the difference in heat resistance between families of olive flounder Paralichthys olivaceus and turbot Scophthalmus maximus, two flatfish species occupying slightly different thermal niches. Then the chosen families were tested to determine their differential response to heat stress (Delta T = +8 degrees C and +12 degrees C) in blood, including anaerobic metabolism (lactate), oxidative stress (lipid peroxidation and protein carbonylation) and antioxidant enzymes. Results showed a difference in heat resistance between families of the two species. Among the chosen parameters, growth traits had a significant effect on contents of lactate and malondialdehyd (MDA), and activities of catalase (CAT) and glutathione S-transferase in flounder (P < 0.05), and on MDA content and CAT activity in turbot (P < 0.05). In comparison with heat-sensitive family of each species, levels of all studied parameters were lower and more stable in heat-resistant families after heat stress. What's more, heat resistance of fish significantly influenced contents of lactate and MDA and activity of CAT in flounder (P < 0.05), as well as contents of lactate, MDA and carbonyl and activity of superoxide dismutate (SOD) in turbot (P < 0.05). These results demonstrated that such physiological phenotypes as anaerobic metabolism, oxidative stress and antioxidant enzymes are good biomarkers of fish heat-resistance, being potentially valuable in fish breeding. However, these markers should be applied with more caution when there is a growth discrepancy between fish families. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：125 / 135

页数：11

共 59 条

[11] Oxidative stress biomarkers in two resident species, mullet (Mugil cephalus) and flounder (Platichtkys flesus), from a polluted site in River Douro Estuary, Portugal [J].