Drosophila melanogaster p24 trafficking proteins have vital roles in development and reproduction

被引：18

作者：

Saleem, Sehresh ^{[1
]}

Schwedes, Christoph C. ^{[1
]}

Ellis, Lisa L. ^{[1
]}

Grady, Stephanie T. ^{[1
]}

Adams, Rachel L. ^{[1
]}

Johnson, Natalie ^{[1
]}

Whittington, Julie R. ^{[1
]}

Carney, Ginger E. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA

来源：

MECHANISMS OF DEVELOPMENT | 2012年 / 129卷 / 5-8期

基金：

美国国家科学基金会;

关键词：

p24; Oviposition; Fertility; Intracellular trafficking; Drosophila melanogaster; Secretory pathway; COATED TRANSPORT VESICLES; PUTATIVE CARGO RECEPTORS; EARLY SECRETORY PATHWAY; TRANSMEMBRANE PROTEIN; ENDOPLASMIC-RETICULUM; MEMBRANE-PROTEIN; GOLGI TRANSPORT; MUTATION CAUSES; FAMILY-MEMBERS; GENE;

D O I：

10.1016/j.mod.2012.04.002

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

p24 proteins comprise a family of type-I transmembrane proteins of similar to 24 kD that are present in yeast and plants as well as metazoans ranging from Drosophila to humans. These proteins are most commonly localized to the endoplasmic reticulum (ER)-Golgi interface and are incorporated in anterograde and retrograde transport vesicles. Little is known about how disruption of p24 signaling affects individual tissue function or whole animals. Drosophila melanogaster express nine p24 genes, grouped into four subfamilies. Based upon our mRNA and protein expression data, Drosophila p24 family members are expressed in a variety of tissues. To identify functions for particular Drosophila p24 proteins, we used RNA interference (RNAi) to reduce p24 expression. Ubiquitous reduction of most p24 genes resulted in complete or partial lethality during development. We found that reducing p24 levels in adults caused defects in female fecundity (egg laying) and also reduced male fertility. We attributed reduced female fecundity to decreased neural p24 expression. These results provide the first genetic analysis of all p24 family members in a multicellular animal and indicate vital roles for Drosophila p24s in development and reproduction, implicating neural expression of p24s in the regulation of female behavior. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：177 / 191

页数：15

共 65 条

[1]

Anantharaman V., 2002, GENOME BIOL, V3

[2] Drosophila p24 homologues eclair and baiser are necessary for the activity of the maternally expressed Tkv receptor during early embryogenesis [J].

Bartoszewski, S ;

Luschnig, S ;

Desjeux, I ;

Grosshans, J ;

Nüsslein-Volhard, C .

MECHANISMS OF DEVELOPMENT, 2004, 121 (10) :1259-1273

[3] Cloning of the gene encoding a novel integral membrane protein, mucolipidin - and identification of the two major founder mutations causing mucolipidosis type IV [J].

Bassi, MT ;

Manzoni, M ;

Monti, E ;

Pizzo, MT ;

Ballabio, A ;

Borsani, G .

AMERICAN JOURNAL OF HUMAN GENETICS, 2000, 67 (05) :1110-1120

[4] Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport [J].

Belden, WJ ;

Barlowe, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (43) :26939-26946

[5] Proteomics characterization of abundant Golgi membrane proteins [J].

Bell, AW ;

Ward, MA ;

Blackstock, WP ;

Freeman, HNM ;

Choudhary, JS ;

Lewis, AP ;

Chotai, D ;

Fazel, A ;

Gushue, JN ;

Paiement, J ;

Palcy, S ;

Chevet, E ;

Lafrenière-Roula, M ;

Solari, R ;

Thomas, DY ;

Rowley, A ;

Bergeron, JJM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (07) :5152-5165

[6] The luminal domain of p23 (Tmp21) plays a critical role in p23 cell surface trafficking [J].

Blum, Robert ;

Lepier, Alexandra .

TRAFFIC, 2008, 9 (09) :1530-1550

[7] Drosophila melanogaster p24 genes have developmental, tissue-specific, and sex-specific expression patterns and functions [J].

Boltz, Kara A. ;

Ellis, Lisa L. ;

Carney, Ginger E. .

DEVELOPMENTAL DYNAMICS, 2007, 236 (02) :544-555

[8] Loss of p24 function in Drosophila melanogaster causes a stress response and increased levels of NF-κB-regulated gene products [J].

Boltz, Kara A. ;

Carney, Ginger E. .

BMC GENOMICS, 2008, 9 (1)

[9] Mucopolysaccharidosis type VI (Maroteaux-Lamy syndrome):: A Y210C mutation causes either altered protein handling or altered protein function of N-acetylgalactosamine 4-sulfatase at multiple points in the vacuolar network [J].

Bradford, TM ;

Litjens, T ;

Parkinson, EJ ;

Hopwood, JJ ;

Brooks, DA .

BIOCHEMISTRY, 2002, 41 (15) :4962-4971

[10]

BRAND AH, 1993, DEVELOPMENT, V118, P401

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