Exercise training enhances cardiac IGFI-R/PI3K/Akt and Bcl-2 family associated pro-survival pathways in streptozotocin-induced diabetic rats

被引：82

作者：

Cheng, Shiu-Min ^{[1
]}

Ho, Tsung-Jung ^{[2
,3
]}

Yang, Ai-Lun ^{[4
]}

Chen, I-Ju ^{[5
]}

Kao, Chung-Lan ^{[6
,7
]}

Wu, Fan-Ni ^{[8
]}

Lin, James A. ^{[8
]}

Kuo, Chia-Hua ^{[9
,10
]}

Ou, Hsiu-Chung ^{[10
]}

Huang, Chih-Yang ^{[2
,8
,11
]}

Lee, Shin-Da ^{[10
,12
]}

机构：

[1] Asia Univ, Dept Psychol, Taichung, Taiwan

[2] China Med Univ, Coll Chinese Med, Sch Chinese Med, Taichung 40202, Taiwan

[3] China Med Univ, Beigang Hosp, Chinese Med Dept, Taichung 40202, Yunlin County, Taiwan

[4] Taipei Phys Educ Coll, Dept Sports Sci, Taipei, Taiwan

[5] Asia Univ, Dept Phys Educ, Taichung, Taiwan

[6] Taipei Vet Gen Hosp, Dept Phys Med & Rehabil, Taipei, Taiwan

[7] Natl Yang Ming Univ, Taipei 112, Taiwan

[8] China Med Univ, Grad Inst Basic Med Sci, Taichung 40202, Taiwan

[9] Taipei Phys Educ Coll, Lab Exercise Biochem, Taipei, Taiwan

[10] China Med Univ, Grad Inst Rehabil Sci, Dept Phys Therapy, Taichung 40202, Taiwan

[11] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, Taiwan

[12] Asia Univ, Dept Healthcare Adm, Taichung, Taiwan

来源：

INTERNATIONAL JOURNAL OF CARDIOLOGY | 2013年 / 167卷 / 02期

关键词：

Apoptosis; Bcl-2; Diabetes; Heart; IGFI-R/PI3K/Akt survival pathway; GROWTH-FACTOR-I; CARDIOMYOCYTE APOPTOSIS; HEART-FAILURE; PHYSICAL-ACTIVITY; CYTOCHROME-C; INSULIN; HYPERTROPHY; KINASE; SKELETAL; DEATH;

D O I：

10.1016/j.ijcard.2012.01.031

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Increased myocyte apoptosis in diabetic hearts has been previously reported. The purpose of this study was to evaluate the effects of exercise training on cardiac survival pathways in streptozotocin (STZ)-induced diabetic rats. Methods: Forty-eight male Wistar rats were randomly divided into control group (Control), STZ-induced (65 mg/kg, i.p.) diabetes (DM), and DM rats with moderate aerobic exercise training (DM-EX) on a treadmill 60 min/day, 5 days/week, for 10 weeks. Histopathological analysis, positive TUNEL assays and Western blotting were performed on the excised cardiac left ventricles from all three groups. Results: The components of cardiac survival pathway (insulin-like growth factor I (IGFI), IGFI-receptor (IGFI-R), phosphatidylinositol 3'-kinase (PI3K), and Akt) and the pro-survival Bcl-2 family proteins (Bcl-2, Bcl-xL, and p-BAD) were all significantly decreased in the DM group compared with the Control group whereas they were increased in the DM-EX group. In addition, the abnormal myocardial architecture, enlarged interstitial space and increased cardiac TUNEL-positive apoptotic cells were observed in the DM group, but they were reduced in the DM-EX group. The apoptotic key component, caspase-3, was significantly increased in the DM group relative to the Control group whereas it was decreased in the DM-EX group. Conclusions: Exercise training enhances cardiac IGFI-R/PI3K/Akt and Bcl-2 family associated pro-survival pathways, which provides one of the new beneficial effects for exercise training in diabetes. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：478 / 485

页数：8

共 37 条

[1] Hypertensive end-organ damage and premature mortality are p38 mitogen-activated protein kinase-dependent in a rat model of cardiac hypertrophy and dysfunction [J].

Behr, TM ;

Nerurkar, SS ;

Nelson, AH ;

Coatney, RW ;

Woods, TN ;

Sulpizio, A ;

Chandra, S ;

Brooks, DP ;

Kumar, S ;

Lee, JC ;

Ohlstein, EH ;

Angermann, CE ;

Adams, JL ;

Sisko, J ;

Sackner-Bernstein, JD ;

Willette, RN .

CIRCULATION, 2001, 104 (11) :1292-1298

[2] Molecular distinction between physiological and pathological cardiac hypertrophy: Experimental findings and therapeutic strategies [J].

Bernardo, Bianca C. ;

Weeks, Kate L. ;

Pretorius, Lynette ;

McMullen, Julie R. .

PHARMACOLOGY & THERAPEUTICS, 2010, 128 (01) :191-227

[3]

Bishopric Nanette H., 2001, Current Opinion in Pharmacology, V1, P141, DOI 10.1016/S1471-4892(01)00032-7

[4] Mitochondrial uncoupling: A key contributor to reduced cardiac efficiency in diabetes [J].

Boudina, Sihem ;

Abel, E. Dale .

PHYSIOLOGY, 2006, 21 :250-258

[5] Nitric oxide, cytochrome c and mitochondria [J].

Brown, GC ;

Borutaite, V .

MITOCHONDRIA AND CELL DEATH, 1999, 66 :17-25

[6]

Colberg SR, 2007, CURR DIABETES REV, V3, P176

[7] Cardiovascular control in experimental diabetes [J].

De Angelis, K ;

Schaan, BD ;

Maeda, CY ;

Dall'Ago, P ;

Wichi, RB ;

Irigoyen, MC .

BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2002, 35 (09) :1091-1100

[8] Effects of Exercise Training on Health Status in Patients With Chronic Heart Failure HF-ACTION Randomized Controlled Trial [J].

Flynn, Kathryn E. ;

Pina, Ileana L. ;

Whellan, David J. ;

Lin, Li ;

Blumenthal, James A. ;

Ellis, Stephen J. ;

Fine, Lawrence J. ;

Howlett, Jonathan G. ;

Keteyian, Steven J. ;

Kitzman, Dalane W. ;

Kraus, William E. ;

Miller, Nancy Houston ;

Schulman, Kevin A. ;

Spertus, John A. ;

O'Connor, Christopher M. ;

Weinfurt, Kevin P. .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2009, 301 (14) :1451-1459

[9] Myocardial cell death in human diabetes [J].

Frustaci, A ;

Kajstura, J ;

Chimenti, C ;

Jakoniuk, I ;

Leri, A ;

Maseri, A ;

Nadal-Ginard, B ;

Anversa, P .

CIRCULATION RESEARCH, 2000, 87 (12) :1123-1132

[10] Cardiomyocyte apoptosis induced by short-term diabetes requires mitochondrial GSH depletion [J].

Ghosh, S ;

Pulinilkunnil, T ;

Yuen, G ;

Kewalramani, G ;

An, D ;

Qi, D ;

Abrahani, A ;

Rodrigues, B .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2005, 289 (02) :H768-H776

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