Temperature effect on the separation of DNA fragments by capillary electrophoresis in hydroxyethyl cellulose solution

被引：0
作者：
Lin, WC
Wen, YM
Hu, JY
Kuei, CH [1 ]
机构：
[1] Natl Cheng Kung Univ, Dept Chem, Tainan 70101, Taiwan
[2] Chia Nan Coll Pharm & Sci, Dept Appl Chem, Tainan, Taiwan
来源：
JOURNAL OF MICROCOLUMN SEPARATIONS | 1999年 / 11卷 / 06期
关键词：
DNA fragment; capillary electrophoresis; hydroxyethyl cellulose; activation energy;
D O I：
10.1002/(SICI)1520-667X(1999)11:6<461::AID-MCS8>3.0.CO;2-D
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The temperature effect on DNA fragment separation in hydroxyethyl cellulose (HEC) media was investigated. The migration time was shorter, but the separation efficiency was improved at elevated temperature for Phi X174/HaeIII separation. The closed 271/281 base pair was successful separated at 55 degrees C in both 0.7 and 1.0% HEC. The activation energy tendency for migration of different fragments showed that there were significant changes for fragments in the 200-400 base range in 1.0% HEC and in the 400-600 base range in 0.7% HEC. (C) 1999 John Wiley & Sons, Inc.
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页码：461 / 470
页数：10
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