Higher Circulating miR-199a-5p Indicates Poor Aerobic Exercise Capacity and Associates With Cardiovascular Dysfunction During Chronic Exposure to High Altitude

被引：6

作者：

Huang, He ^{[1
,2
,3
,4
]}

Xie, Shenwei ^{[5
]}

Gu, Xiaolan ^{[6
]}

Xiang, Bin ^{[1
,3
,4
]}

Zhong, Zhifeng ^{[1
,3
,4
]}

Huang, Pei ^{[1
,3
,4
]}

Gao, Yuqi ^{[2
,3
,4
]}

Li, Peng ^{[1
,3
,4
]}

机构：

[1] Third Mil Med Univ, Amry Med Univ, Coll High Altitude Mil Med, Dept High Altitude Operat Med, Chongqing, Peoples R China

[2] Third Mil Med Univ, Amry Med Univ, Inst Med & Equipment High Altitude Reg, Coll High Altitude Mil Med, Chongqing, Peoples R China

[3] Minist Educ China, Key Lab Extreme Environm Med, Chongqing, Peoples R China

[4] PLA, Key Lab High Altitude Med, Chongqing, Peoples R China

[5] Third Mil Med Univ, Army Med Univ, Xinqiao Hosp, Shigatse Branch,Affiliated Hosp 2, Chongqing, Tibet, Peoples R China

[6] Third Mil Med Univ, Army Med Univ, Affiliated Hosp 1, Dept Infect Dis, Chongqing, Peoples R China

来源：

FRONTIERS IN PHYSIOLOGY | 2021年 / 12卷

关键词：

circulating microRNA; miR-199a-5p; biomarker; cardiovascular dysfunction; exercise capacity; hypoxia; high altitude;

D O I：

10.3389/fphys.2021.587241

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Background Hypoxia-induced decline in exercise capacity is ubiquitous among lowlanders who immigrated to high altitudes, which severely reduces their work efficiency and quality of life. Although studies have revealed that hypoxia-induced cardiovascular dysfunction limits exercise capacity at high altitudes, the mechanisms have not been well explored at the molecular level. miR-199a-5p is hypoxia-sensitive and serves as an important regulator in cardiovascular pathophysiology. However, whether miR-199a-5p is involved in cardiovascular dysfunction at high altitudes and contributes to subsequent reductions in exercise capacity remains unknown. Thus, this study aimed at exploring these relationships in a high altitude population. Methods A total of 175 lowlanders who had immigrated to an altitude of 3,800 m 2 years previously participated in the present study. The level of plasma miR-199a-5p and the concentration of serum myocardial enzymes were detected by qRT-PCR and ELISA, respectively. Indices of cardiovascular function were examined by echocardiography. The exercise capacity was evaluated by Cooper's 12-min run test and the Harvard Step Test. Furthermore, we explored the biological functions of miR-199a-5p with silico analysis and a biochemical test. Results The level of miR-199a-5p was significantly higher in individuals with poor exercise capacity at 3,800 m, compared with those with good exercise capacity (p < 0.001). miR-199a-5p accurately identified individuals with poor exercise capacity (AUC = 0.752, p < 0.001). The level of miR-199a-5p was positively correlated with cardiovascular dysfunction indices (all, p < 0.001). Furthermore, miR-199a-5p was involved in the oxidative stress process. Conclusion In this study, we reported for the first time that the level of circulating miR-199a-5p was positively associated with exercise capacity during chronic hypoxia at high altitudes. Moreover, higher miR-199a-5p was involved in hypoxia-induced cardiovascular dysfunctions, thus contributing to poorer exercise endurance at high altitudes.

引用

页数：12