Trypanosoma cruzi: Multiplex PCR to detect and classify strains according to groups I and II

被引：12

作者：

Liarte, Daniel B. ^{[1
]}

Murta, Silvane M. F. ^{[1
]}

Steindel, Mario ^{[2
]}

Romanha, Alvaro J. ^{[1
]}

机构：

[1] Fiocruz MS, Ctr Pesquisas Rene Rachou, Lab Parasitol Celular & Mol, BR-30190002 Belo Horizonte, MG, Brazil

[2] Univ Fed Santa Catarina, Dept Microbiol & Parasitol, BR-88040900 Florianopolis, SC, Brazil

来源：

EXPERIMENTAL PARASITOLOGY | 2009年 / 123卷 / 04期

关键词：

Trypanosoma cruzi; Satellite DNA; Multiplex PCR; Molecular diagnosis; T. cruzi group; Triatomine; Triatoma infestans; rDNA; ribosomal DNA; DNA; deoxyribonucleic acid; kDNA; kinetoplast deoxyribonucleic acid; PBS; phosphate buffered saline; PCR; polymerase chain reaction; RAPD; random amplification of polymorphic; RFLP; restriction fragment length polymorphism; T. cruzi I; Trypanosoma cruzi group I; T cruzi II; Trypanosoma cruzi group II; Z1; Z2; Z3; zymodemes; 1; 2; and; 3; AMPLIFIED POLYMORPHIC DNA; PHYLOGENETIC LINEAGES; TRIATOMINE BUGS; SANTA-CATARINA; REPETITIVE DNA; SATELLITE DNA; IDENTIFICATION; INFECTION; VECTORS; STATE;

D O I：

10.1016/j.exppara.2008.12.005

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A multiplex PCR was developed for simultaneous detection of Trypanosoma cruzi DNA and classification of the parasite strain into groups I and II. As little as 10 fg of T. cruzi DNA could be detected by multiplex PCR. The technique was shown to be specific for T. cruzi DNA, since no PCR amplification products were obtained with DNA from other tripanosomatid species. Multiplex PCR was validated by assaying genomic DNA from 34 strains of T. cruzi that had been previously characterized; 24 blood samples from experimentally-infected mice and non-infected controls; 20 buffy coat samples from patients in the acute phase of Chagas disease and non-infected individuals, and 15 samples of feces from naturally-infected Triatoma infestans. T. cruzi samples from patients and from Y strain-infected mice were classified by multiplex PCR as T cruzi II and samples from T. infestans and Colombiana strain-infected mice as T. cruzi I. (c) 2009 Elsevier Inc. All rights reserved.

引用

页码：283 / 291

页数：9

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