Characterization of an influenza A H5N2 reassortant as a candidate for live-attenuated and inactivated vaccines against highly pathogenic H5N1 viruses with pandemic potential

被引：41
作者：
Desheva, J. A.
Lu, X. H.
Rekstin, A. R.
Rudenko, L. G.
Swayne, D. E.
Cox, N. J.
Katz, J. M.
Klimov, A. I.
机构：
[1] Ctr Dis Control & Prevent, Influenza Branch, Atlanta, GA 30333 USA
[2] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA
[3] Russian Acad Med Sci, Inst Expt Med, Dept Virol, St Petersburg, Russia
来源：
VACCINE | 2006年 / 24卷 / 47-48期
关键词：
avian influenza vaccine; influenza pandemic; mouse model;
D O I：
10.1016/j.vaccine.2006.06.023
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We generated a high-growth 7:1 reassortant (Len17/H5) that contained the hemagglutinin (HA) gene from non-pathogenic A/Duck/Potsdam/1402-6/86 (H5N2) virus and other genes from the cold-adapted (ca) attenuated A/leningrad/134/17/57 (H2H2) strain. Len17/H5 demonstrated an attenuated phenotype in mice and did not infect chickens. Mice administered Len17/H5 either as a live-attenuated intranasal vaccine or as an inactivated intramuscular vaccine were substantially protected from lethal challenge with highly pathogenic A/Hong Kong/483/97 (H5N1) vir-us and were protected from pulmonary infection with antigenically distinct A/Hong Kong/213/2003 (H5N1) virus. The cross-protective effect correlated with the levels of virus-specific mucosal IgA and/or serum IgG antibodies. Our results suggest a new strategy of using classical genetic reassortment between a high-growth ca H2N2 strain and antigenically related non-pathogenic avian viruses to prepare live-attenuated and inactivated vaccines for influenza pandemic. Published by Elsevier Ltd.
引用
收藏
页码：6859 / 6866
页数：8
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