Hyperosmotic chemical agent's effect on in vivo cerebral blood flow revealed by laser speckle

被引：27

作者：

Cheng, HY ^{[1
]}

Luo, QM ^{[1
]}

Zeng, SQ ^{[1
]}

Chen, SB ^{[1
]}

Luo, WH ^{[1
]}

Gong, H ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Minist Educ, Key Lab Biomed Photon, Wuhan 430074, Hubei, Peoples R China

来源：

APPLIED OPTICS | 2004年 / 43卷 / 31期

关键词：

D O I：

10.1364/AO.43.005772

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We investigated the influence of a hyperosmotic agent (glycerol) on the normal physiological function of tissue by applying the glycerol in vitro and in vivo to rabbit dura mater to assess the changes in the tissue's optical properties. We used a laser speckle imaging technique to study the effect of epidurally 49 applied glycerol on resting cerebral blood flow (CBF). Our results showed that resting CBF decreased as the transparency of the dura mater increased. The challenges for the design of an optical clearing technique were not only the clearing effects and the duration of the action of the chemical agents but also the influence of the glycerol on the tissue's normal physiological function. (C) 2004 Optical Society of America.

引用

页码：5772 / 5777

页数：6

共 31 条

[1]

Bakutkin V.V., 1987, J APPL SPECTROSC+, V46, P104, DOI [10.1007/BF00660291, DOI 10.1007/BF00660291]

[2] Glucose and mannitol diffusion in human dura mater [J].

Bashkatov, AN ;

Genina, EA ;

Sinichkin, YP ;

Kochubey, VI ;

Lakodina, NA ;

Tuchin, VV .

BIOPHYSICAL JOURNAL, 2003, 85 (05) :3310-3318

[3] In vitro study of control of human dura mater optical properties by acting of osmotical liquids [J].

Bashkatov, AN ;

Genina, EA ;

Kochubey, VI ;

Sinichkin, YP ;

Korobov, AA ;

Lakodina, NA ;

Tuchin, VV .

CONTROLLING TISSUE OPTICAL PROPERTIES: APPLICATIONS IN CLINICAL STUDY, 2000, 4162 :182-188

[4]

BASHKATOV VV, 1995, P SOC PHOTO-OPT INS, V2393, P137

[5] Intrinsic brain activity triggers trigeminal meningeal afferents in a migraine model [J].

Bolay, H ;

Reuter, U ;

Dunn, AK ;

Huang, ZH ;

Boas, DA ;

Moskowitz, MA .

NATURE MEDICINE, 2002, 8 (02) :136-142

[6] MODEL FOR LASER DOPPLER MEASUREMENTS OF BLOOD-FLOW IN TISSUE [J].

BONNER, R ;

NOSSAL, R .

APPLIED OPTICS, 1981, 20 (12) :2097-2107

[7]

Briers J D, 1996, J Biomed Opt, V1, P174, DOI 10.1117/12.231359

[8]

CHAN E, 1996, IEEE J SEL TOP QUANT, P943

[9] A chamber and artificial dura method for long-term optical imaging in the monkey [J].

Chen, LM ;

Heider, B ;

Williams, GV ;

Healy, FL ;

Ramsden, BM ;

Roe, AW .

JOURNAL OF NEUROSCIENCE METHODS, 2002, 113 (01) :41-49

[10] Efficient characterization of regional mesenteric blood flow by use of laser speckle imaging [J].

Cheng, HY ;

Luo, QM ;

Wang, Z ;

Gong, H ;

Chen, SB ;

Liang, WX ;

Zeng, SQ .

APPLIED OPTICS, 2003, 42 (28) :5759-5764

← 1 2 3 4 →