Fourteen polymorphic microsatellite DNA markers for the human malaria parasite Plasmodium vivax

被引：79

作者：

Karunaweera, N. D.

Ferreira, M. . U.

Hartl, D. . L.

Wirth, D. F.

机构：

[1] Harvard Univ, Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA

[2] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA

来源：

MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 01期

关键词：

genetic diversity; malaria; microsatellites; Plasmodium vivax; polymorphism; Sri Lanka;

D O I：

10.1111/j.1471-8286.2006.01534.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have optimized a set of 14 polymorphic microsatellite markers for the human malaria parasite Plasmodium vivax, all of them consisting of either tri- or tetranucleotide repeats. These markers, whose polymerase chain reaction amplification conditions are identical, were used to screen 25 parasite isolates from malaria-endemic areas in Sri Lanka. The total number of alleles per locus ranged between 6 and 13 (average, 7.8), and expected heterozygosity ranged from 0.627 to 0.913 (average, 0.790). These markers are now being used to characterize the population structure of P. vivax in other endemic areas.

引用

页码：172 / 175

页数：4