Fourteen polymorphic microsatellite DNA markers for the human malaria parasite Plasmodium vivax

被引：79
作者：
Karunaweera, N. D.
Ferreira, M. . U.
Hartl, D. . L.
Wirth, D. F.
机构：
[1] Harvard Univ, Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA
[2] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA
来源：
MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 01期
关键词：
genetic diversity; malaria; microsatellites; Plasmodium vivax; polymorphism; Sri Lanka;
D O I：
10.1111/j.1471-8286.2006.01534.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have optimized a set of 14 polymorphic microsatellite markers for the human malaria parasite Plasmodium vivax, all of them consisting of either tri- or tetranucleotide repeats. These markers, whose polymerase chain reaction amplification conditions are identical, were used to screen 25 parasite isolates from malaria-endemic areas in Sri Lanka. The total number of alleles per locus ranged between 6 and 13 (average, 7.8), and expected heterozygosity ranged from 0.627 to 0.913 (average, 0.790). These markers are now being used to characterize the population structure of P. vivax in other endemic areas.
引用
收藏
页码：172 / 175
页数：4
相关论文
共 12 条
[1]   Complex mutations in a high proportion of microsatellite loci from the protozoan parasite Plasmodium falciparum [J].
Anderson, TJC ;
Su, XZ ;
Roddam, A ;
Day, KP .
MOLECULAR ECOLOGY, 2000, 9 (10) :1599-1608
[2]   Microsatellite markers reveal a spectrum of population structures in the malaria parasite Plasmodium falciparum [J].
Anderson, TJC ;
Haubold, B ;
Williams, JT ;
Estrada-Franco, JG ;
Richardson, L ;
Mollinedo, R ;
Bockarie, M ;
Mokili, J ;
Mharakurwa, S ;
French, N ;
Whitworth, J ;
Velez, ID ;
Brockman, AH ;
Nosten, F ;
Ferreira, MU ;
Day, KP .
MOLECULAR BIOLOGY AND EVOLUTION, 2000, 17 (10) :1467-1482
[3]   Tandem repeats finder: a program to analyze DNA sequences [J].
Benson, G .
NUCLEIC ACIDS RESEARCH, 1999, 27 (02) :573-580
[4]   The Plasmodium vivax genome sequencing project [J].
Carlton, J .
TRENDS IN PARASITOLOGY, 2003, 19 (05) :227-231
[5]   The genetic diversity of Plasmodium vivax populations [J].
Cui, LW ;
Escalante, AA ;
Imwong, M ;
Snounou, G .
TRENDS IN PARASITOLOGY, 2003, 19 (05) :220-226
[6]   Comprehensive human genome amplification using multiple displacement amplification [J].
Dean, FB ;
Hosono, S ;
Fang, LH ;
Wu, XH ;
Faruqi, AF ;
Bray-Ward, P ;
Sun, ZY ;
Zong, QL ;
Du, YF ;
Du, J ;
Driscoll, M ;
Song, WM ;
Kingsmore, SF ;
Egholm, M ;
Lasken, RS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) :5261-5266
[7]   Identification of a polymorphic Plasmodium vivax microsatellite marker [J].
Gomez, JC ;
McNamara, DT ;
Bockarie, MJ ;
Baird, JK ;
Carlton, JM ;
Zimmerman, PA .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2003, 69 (04) :377-379
[8]   Microsatellite variation, repeat array length, and population history of plasmodium vivax [J].
Imwong, M ;
Sudimack, D ;
Pukrittayakamee, S ;
Osorio, L ;
Carlton, JM ;
Day, NPJ ;
White, NJ ;
Anderson, TJC .
MOLECULAR BIOLOGY AND EVOLUTION, 2006, 23 (05) :1016-1018
[9]   Meager genetic variability of the human malaria agent Plasmodium vivax [J].
Leclerc, MC ;
Durand, P ;
Gauthier, C ;
Patot, S ;
Billotte, N ;
Menegon, M ;
Severini, C ;
Ayala, FJ ;
Renaud, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (40) :14455-14460
[10]   Genetic structure of Plasmodium falciparum populations in the Brazilian Amazon region [J].
Machado, RLD ;
Póvoa, MM ;
Calvosa, VSP ;
Ferreira, MU ;
Rossit, ARB ;
dos Santos, EJM ;
Conway, DJ .
JOURNAL OF INFECTIOUS DISEASES, 2004, 190 (09) :1547-1555
12