Terrilactibacillus laevilacticus gen. nov., sp nov., isolated from soil

A Gram-stain-positive, catalase-positive, facultatively anaerobic, spore-forming, rod-shaped bacterium, strain NK26-11(T), was isolated from soil in Thailand. This strain produced D-lactic acid from glucose homofermentatively, and grew at 20-45 degrees C and pH 5-8.5. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major respiratory quinone was menaquinone 7 (MK-7), the DNA G + C content was 42.6 mol%, and the major cellular fatty acids were anteiso-C-15 : 0 and anteiso-C-17 : 0. On the basis of 16S rRNA gene sequences analysis, strain NK26-11(T) was closely related to Bacillus solimangrovi JCM 18994(T) (93.89 % 16S rRNA gene sequence similarity), Pullulanibacillus naganoensis LMG 12887(T) (93.32 %), Sporolactobacillus inulinus NRIC 1133(T) (92.99 %), Tuberibacillus calidus JCM 13397(T) (92.98 %) and Thalassobacillus devorans DSM 16966(T) (<90.93 %). Strain NK26-11(T) could be clearly distinguished from the closely related genera based on phenotypic characteristics and DNA G + C content, and thus represents a novel species of a new genus between the Bacillus and Sporolactobacillus cluster, for which the name Terrilactibacillus laevilacticus gen. nov., sp. nov. is proposed. The type strain of the type species is NK26-11(T) (=LMG 27803(T)=TISTR 2241(T)=PCU 335(T)).