Transduction of TAT fusion proteins into osteoclasts and osteoblasts

被引:20
作者
Dolgilevich, S
Zaidi, N
Song, JB
Abe, E
Moonga, BS
Sun, L
机构
[1] CUNY Mt Sinai Sch Med, Mt Sinai Bone Program, New York, NY 10029 USA
[2] CUNY Mt Sinai Sch Med, Dept Med, New York, NY 10029 USA
[3] Bronx Vet Affairs Med Ctr, GRECC, New York, NY 10029 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1016/S0006-291X(02)02664-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It has been difficult to transduce primary cultures of bone cells with proteins of interest. Here, we report the development and validation of a new technology for transduction of osteoblasts and osteoclasts with peptides and moderately sized proteins. Fusion proteins between TAT, an 11 amino acid Arg-rich sequence derived from the HIV protein, and either hemagglutinin or calcineurin Aalpha were synthesized and purified. Exposure of osteoblasts and osteoclasts in primary culture to either TAT-HA or TAT-calcineurin Aalpha resulted in a rapid (within 10 min) intracellular movement of the fusion protein evident on co-immunostaining. Almost 99% of cells were transduced and the fusion protein was retained in similar to50% of the cells for up to 5 days. TAT did not abolish the functionality of calcineurin Aalpha; the fusion protein stimulated osteoblast differentiation and inhibited osteoclastic resorption. We expect that our studies will provide a firm basis for the future development of TAT fusion proteins for critical molecules involved in bone cell differentiation and function. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:505 / 509
页数:5
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