Methods for purifying and detoxifying sodium dodecyl sulfate-stabilized polyacrylate nanoparticles

被引：30

作者：

Garay-Jimenez, Julio C. ^{[1
]}

Young, Ashley ^{[2
]}

Gergeres, Danielle ^{[1
]}

Greenhalgh, Kerriann ^{[1
]}

Turos, Edward ^{[1
]}

机构：

[1] Univ S Florida, Dept Chem, Ctr Mol Divers Drug Design Discovery & Delivery, Tampa, FL 33620 USA

[2] Nanopharma Technol Inc, Tampa, FL USA

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2008年 / 4卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

Polyacrylate nanoparticles; Emulsions; Cytotoxicity; Sodium dodecyl sulfate;

D O I：

10.1016/j.nano.2008.03.004

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Recent research in our laboratory has centered on studies of polyacrylate and polyacrylamide nanoparticle emulsions for use in antibiotic delivery. Our goal is to develop these nanoparticle emulsions for treatment of life-threatening bacterial infections such as those caused by methicillin-resistant Staphylococcus aureus. For this intended application it is necessary to ensure that the biological activity of the emulsion is due only to the drug attached to the polymeric chain and not to any extraneous components. To investigate this we evaluated cytotoxicity and microbiological activity of the nanoparticle emulsions before and after purification by centrifugation, dialysis, and gel filtration. Depending on the amount of surfactant used, all or most of the microbial and cellular toxicity can be removed by a simple purification procedure. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：98 / 105

页数：8

共 28 条

[1] Glycosylated polyacrylate nanoparticles by emulsion polymerization [J].

Abeylath, Sampath C. ;

Turos, Edward .

CARBOHYDRATE POLYMERS, 2007, 70 (01) :32-37

[2]

ALLEMANN E, 1993, EUR J PHARM BIOPHARM, V39, P13

[3] Sodium lauryl sulfate and triclosan: In vitro cytotoxicity studies with gingival cells [J].

Babich, H ;

Babich, JP .

TOXICOLOGY LETTERS, 1997, 91 (03) :189-196

[4] SEPARATION OF DRUG-LOADED NANOPARTICLES FROM FREE DRUG BY GEL-FILTRATION [J].

BECK, P ;

SCHERER, D ;

KREUTER, J .

JOURNAL OF MICROENCAPSULATION, 1990, 7 (04) :491-496

[5]

CRAIG S, 2000, J INVEST DERMATOL, V115, P292

[6] Comparison of diafiltration and tangential flow filtration for purification of nanoparticle suspensions [J].

Dalwadi, G ;

Benson, HAE ;

Chen, Y .

PHARMACEUTICAL RESEARCH, 2005, 22 (12) :2152-2162

[7] The price of innovation: new estimates of drug development costs [J].

DiMasi, JA ;

Hansen, RW ;

Grabowski, HG .

JOURNAL OF HEALTH ECONOMICS, 2003, 22 (02) :151-185

[8] Is there hope for the prevention of future antimicrobial shortages? [J].

Gilbert, DN ;

Edwards, JE .

CLINICAL INFECTIOUS DISEASES, 2002, 35 (02) :215-216

[9] Antimicrobial resistance determinants and future control [J].

Harbarth, S ;

Samore, MH .

EMERGING INFECTIOUS DISEASES, 2005, 11 (06) :794-801

[10] Sodium dodecyl sulfate and C31G as microbicidal alternatives to nonoxynol 9: Comparative sensitivity of primary human vaginal keratinocytes [J].

Krebs, FC ;

Miller, SR ;

Catalone, BJ ;

Welsh, PA ;

Malamud, D ;

Howett, MK ;

Wigdahl, B .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (07) :1954-1960

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