The methanolic fraction of Centratherum anthelminticum seed downregulates pro-inflammatory cytokines, oxidative stress, and hyperglycemia in STZ-nicotinamide-induced type 2 diabetic rats

被引：42

作者：

Arya, Aditya ^{[1
,2
]}

Cheah, Shiau Chuen ^{[2
]}

Looi, Chung Yeng ^{[2
]}

Taha, Hairin ^{[2
]}

Mustafa, Mohd Rais ^{[2
]}

Mohd, Mustafa Ali ^{[2
]}

机构：

[1] Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

[2] Univ Malaya, Fac Med, Dept Pharmacol, Kuala Lumpur 50603, Malaysia

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2012年 / 50卷 / 11期

关键词：

Diabetes mellitus; NF-kappa B translocation; Cytokines; Antioxidant; Phenolics; Centratherum anthelminticum seed; CARDIOVASCULAR-DISEASE; TRANSCRIPTION FACTOR; INSULIN-RESISTANCE; PHENOLIC-COMPOUNDS; ACID-DERIVATIVES; CAFFEIC ACID; NEW-MODEL; KAPPA-B; OBESITY; MASS;

D O I：

10.1016/j.fct.2012.08.012

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study aimed to ascertain the potential of Centratherum anthelminticum seeds methanolic fraction (CAMFs) for the management of type 2 diabetes and its associated complications. CAMFs was initially tested on beta-TC6 cells for H2O2-induced nuclear factor-kappa B (NF-kappa B) translocation effects. The result displayed that CAMFs significantly inhibited NF-kappa B translocation from cytoplasm into the nucleus, dose-dependently. Furthermore, a 12-week sub-chronic CAMFs study was carried out on streptozotocin (STZ)-nicotinamide-induced type 2 diabetic rat model to evaluate glycemia, essential biochemical parameters, lipid levels, oxidative stress markers, and pro-inflammatory cytokines level. Our study result showed that CAMFs reduced hyperglycemia by increasing serum insulin, C-peptide, total protein, and albumin levels, significantly. Whereas, elevated blood glucose, glycated hemoglobin, lipids and enzyme activities were restored to near normal. CAMFs confirmed antioxidant potential by elevating glutathione (GSH) and reducing malondialdehyde (MDA) levels in diabetic rats. Interestingly, CAMFs down-regulated elevated tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-1 beta and IL-6 in the tissues and serum of the diabetic rats. We conclude that CAMFs exerted apparent antidiabetic effects and demonstrated as a valuable candidate nutraceutical for insulin-resistant type 2 diabetes and its associated complications such as dyslipidemia, oxidative stress, and inflammation. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：4209 / 4220

页数：12

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