Pyrosequencing reveals microbial community dynamics in integrated simultaneous desulfurization and denitrification process at different influent nitrate concentrations

被引：90

作者：

Chen, Chuan ^{[1
]}

Xu, Xi-Jun ^{[1
]}

Xie, Peng ^{[1
]}

Yuan, Ye ^{[4
]}

Zhou, Xu ^{[1
]}

Wang, Ai-Jie ^{[1
]}

Lee, Duu-Jong ^{[1
,2
,3
]}

Ren, Nan-Qi ^{[1
]}

机构：

[1] Harbin Inst Technol, Sch Municipal & Environm Engn, State Key Lab Urban Water Resource & Environm, POB 2650,73 Huanghe Rd, Harbin 150090, Heilongjiang Pr, Peoples R China

[2] Natl Taiwan Univ, Dept Chem Engn, Taipei 106, Taiwan

[3] Natl Taiwan Univ Sci & Technol, Dept Chem Engn, Taipei 106, Taiwan

[4] Yancheng Inst Technol, Sch Environm Sci & Engn, Yancheng 224051, Peoples R China

来源：

CHEMOSPHERE | 2017年 / 171卷

基金：

中国国家自然科学基金;

关键词：

Pyrosequencing; Microbial community; Integrated simultaneous desulfurization and denitrification; Nitrate; EPSILON-PROTEOBACTERIA; BACTERIAL COMMUNITY; REDUCING BACTERIA; SULFATE-REDUCTION; OIL-FIELD; GEN; NOV; REMOVAL; WATER; DIVERSITY; CHEMOLITHOAUTOTROPH;

D O I：

10.1016/j.chemosphere.2016.11.159

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Integrated simultaneous desulfurization and denitrification (ISDD) process has proven to be feasible for the coremoval of sulfate, nitrate, and chemical oxygen demand (COD). In this study, we aimed to reveal the microbial community dynamics in the ISDD process with different influent nitrate (NO3-) concentrations. For all tested scenarios, full denitrification was accomplished while sulfate removal efficiency decreased along with increased influent NO3- concentrations. The proportion of S-0 to influent SO42- maintained a low level (5.6-17.0%) regardless of the increased influent NO3- concentrations. Microbial community analysis results showed that higher influent NO3- concentrations affected the microbial community structure greatly. Phyla Proteobacteria, Spirochaetae, Firmicutes, Synergistetes, and Chloroflexi dominated in all the community compositions, of which Proteobacteria exhibited a clear difference among eight microbial samples. Members of delta-Proteobacteria, with 16S rRNA gene sequences related to Desulfobulbus, were clearly decreased at influent NO3- = 3000 and 3500 mg/L, suggesting an inhibitory effect of NO3- on sulfate reduction. In contrast, as influent NO3- concentration increased, microbial community was notably enriched in gamma-Proteobacteria and epsilon-Proteobacteria, which revealed the enrichment of 16S rRNA gene sequences related to Pseudomonas (gamma-Proteobacteria), and Arcobacteria and Sulfurospirillum (epsilon-Proteobacteria). (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：294 / 301

页数：8

共 54 条

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