Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China

被引:90
作者
Chen, Jianfei [1 ]
Liu, Xiaozhen [1 ]
Shi, Da [1 ]
Shi, Hongyan [1 ]
Zhang, Xin [1 ]
Li, Changlong [1 ]
Chi, Yanbin [1 ]
Feng, Li [1 ]
机构
[1] Chinese Acad Agr Sci, Div Swine Infect Dis, State Key Lab Vet Biotechnol, Harbin Vet Res Inst, Harbin 150001, Peoples R China
来源
VIRUSES-BASEL | 2013年 / 5卷 / 10期
基金
中国国家自然科学基金;
关键词
PEDV; S gene; heterogeneity; genetic variation; variants; TRANSMISSIBLE GASTROENTERITIS VIRUS; COMPLETE GENOME SEQUENCE; GLYCOPROTEIN GENE; IDENTIFICATION; PROTEIN; STRAIN;
D O I
10.3390/v5102601
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Since late 2010, porcine epidemic diarrhea virus (PEDV) has rapidly disseminated all over the China and caused considerable morbidity and high mortality (up to 100%) in neonatal piglets. 79.66% (141 of 177) pig farms in 29 provinces (excluding Tibet and Hainan, China) and 72.27% (417 of 577) samples were positive for PEDV confirmed by reverse transcription-polymerase chain reaction (RT-PCR). The full-length S genes of representative field strains were sequenced. 33 field strains share 93.5%-99.9% homologies with each other at the nucleotide sequence level and 92.3%-99.8% homologies with each other at the amino acids sequence level. Most field strains have nucleotide deletion and insertion regions, and show lower homologies (93.5%-94.2%) with Chinese classical strain CH/S, however higher homologies (97.1%-99.3%) with recent strain CHGD-1. The phylogenetic analysis showed there are classical strains and variants prevailing in pig herd in China. PEDV has a high detection rate in pig herds in China. Sequence analysis indicated the S genes of recent field strains have heterogeneity and the variants are predominant.
引用
收藏
页码:2601 / 2613
页数:13
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