Type-1 and type-2 cytokines in human late-gestation decidual tissue

被引:63
作者
Jones, CA [1 ]
FinlayJones, JJ [1 ]
Hart, PH [1 ]
机构
[1] FLINDERS UNIV S AUSTRALIA, SCH MED, DEPT MICROBIOL & INFECT DIS, ADELAIDE, SA 5001, AUSTRALIA
关键词
D O I
10.1095/biolreprod57.2.303
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Evidence suggests that successful pregnancy in rodents requires the preferential stimulation of type-2 cytokine production by gestation-associated tissues. The ability of human gestation-associated tissue to synthesize type-2 and type-1 cytokines has not been examined in detail and was the subject of this study. Decidual tissue collected at term, either before the onset of labor (elective caesarean section) or at the completion of labor, expressed mRNA for interleukin (IL)-10 and interferon gamma (IFN gamma) and produced IL-10 and IFN gamma protein in vitro. IL-4 mRNA or protein was not detected. IFN gamma production in vitro was significantly decreased by decidual cells prepared from decidua collected after labor as compared with those collected before the commencement of labor, a change that may be associated with prostaglandin E-2 (PGE(2)) production in conjunction with labor. IFN gamma, IL-4, and IL-10 were detectable in human amniotic fluid collected at term. There was no significant difference in the levels of IL-4, IFN gamma, or IL-10 in samples collected before labor onset or during labor. Thus, late-gestation human decidual tissue and amniotic fluid do not preferentially express a type-2 cytokine profile of cytokines. This is not consistent with observations of a predominant type-2 cytokine profile in gestation-associated tissues in murine pregnancy.
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页码:303 / 311
页数:9
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