Growth-Dependent Catalase Localization in Exiguobacterium oxidotolerans T-2-2T Reflected by Catalase Activity of Cells

被引:11
作者
Hanaoka, Yoshiko [1 ,2 ]
Takebe, Fumihiko [2 ,3 ]
Nodasaka, Yoshinobu [4 ]
Hara, Isao [2 ]
Matsuyama, Hidetoshi [3 ]
Yumoto, Isao [1 ,2 ]
机构
[1] Hokkaido Univ, Lab Environm Microbiol, Grad Sch Agr, Kita Ku, Sapporo, Hokkaido, Japan
[2] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Toyohira Ku, Sapporo, Hokkaido, Japan
[3] Tokai Univ, Sch Biol Sci & Engn, Minami Ku, Sapporo, Hokkaido, Japan
[4] Hokkaido Univ, Grad Sch Dent, Lab Electron Microscopy, Kita Ku, Sapporo, Hokkaido, Japan
来源
PLOS ONE | 2013年 / 8卷 / 10期
关键词
FACULTATIVELY PSYCHROPHILIC BACTERIUM; VIBRIO-RUMOIENSIS S-1(T); HYDROGEN-PEROXIDE; SUPEROXIDE-PRODUCTION; PROTEIN SECRETION; OXIDATIVE STRESS; DNA DAMAGE; PSYCHROBACTER; MECHANISM; H2O2;
D O I
10.1371/journal.pone.0076862
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A psychrotolerant and H2O2-resistant bacterium, Exiguobacterium oxidotolerans T-2-2(T), exhibits extraordinary H2O2 resistance and produces catalase not only intracellularly but also extracellularly. The intracellular and extracellular catalases exhibited the same enzymatic characteristics, that is, they exhibited the temperature-dependent activity characteristic of a cold-adapted enzyme, their heat stabilities were similar to those of mesophilic enzymes and very high catalytic intensity. In addition, catalase gene analysis indicated that the bacterium possessed the sole clade 1 catalase gene corresponding to intracellular catalase. Hence, intracellular catalase is secreted into the extracellular space. In addition to intracellular and extracellular catalases, the inner circumference of the cells showed the localization of catalase in the mid-stationary growth phase, which was observed by immunoelectron microscopy using an antibody against the intracellular catalase of the strain. The cells demonstrated higher catalase activity in the mid-stationary growth phase than in the exponential growth phase. The catalase localized in the inner circumference can be dissociated by treatment with Tween 60. Thus, the localized catalase is not tightly bound to the inner circumference of the cells and may play a role in the oxidative defense of the cells under low metabolic state.
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页数:8
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