A practical guide to quantify cell adhesion using single-cell force spectroscopy

被引：155

作者：

Friedrichs, Jens ^{[1
]}

Legate, Kyle R. ^{[3
]}

Schubert, Rajib ^{[2
]}

Bharadwaj, Mitasha ^{[2
]}

Werner, Carsten ^{[1
]}

Muellner, Daniel J. ^{[2
]}

Benoit, Martin ^{[3
]}

机构：

[1] Leibniz Inst Polymer Res Dresden, Inst Biofunct Polymer Mat, D-01069 Dresden, Germany

[2] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, CH-4058 Basel, Switzerland

[3] Ludwig Maximilians Univ Munchen, D-80799 Munich, Germany

来源：

METHODS | 2013年 / 60卷 / 02期

基金：

瑞士国家科学基金会;

关键词：

AFM; Atomic force microscopy; Cell-cell adhesion; Cell adhesion molecules; Cell surface receptor; Extracellular matrix; Membrane tethers; Receptor-ligand interactions; SCFS; Single-cell force spectroscopy; ACTIN STRESS FIBERS; PLATE FLOW CHAMBER; TRACTION FORCES; ERYTHROCYTE-MEMBRANE; MOLECULAR ADHESION; GLOBAL ASSESSMENT; OPTICAL TWEEZERS; EXTENSIONAL FLOW; FOCAL ADHESIONS; ELASTIC TETHER;

D O I：

10.1016/j.ymeth.2013.01.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantitative analysis of cellular interactions with the extracellular environment is necessary to gain an understanding of how cells regulate adhesion in the development and maintenance of multicellular organisms, and how changes in cell adhesion contribute to diseases. We provide a practical guide to quantify the adhesive strength of living animal cells to various substrates using atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS). We describe how to control cell state and attachment to the AFM cantilever, how to functionalize supports for SCFS measurements, how to conduct cell adhesion measurements, and how to analyze and interpret the recorded SCFS data. This guide is intended to assist newcomers in the field to perform AFM-based SCFS measurements. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：169 / 178

页数：10

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