Effects of dietary cholesterol on astaxanthin transport in plasma of Atlantic salmon (Salmo salar)

被引:27
作者
Chimsung, N. [1 ,2 ]
Lall, S. P. [1 ]
Tantikitti, C. [2 ]
Verlhac-Trichet, V. [3 ]
Milley, J. E. [1 ]
机构
[1] Natl Res Council Canada, Halifax, NS B3H 3Z1, Canada
[2] Prince Songkla Univ, Fac Nat Resources, Dept Aquatic Sci, Hat Yai, Songkhla, Thailand
[3] DSM Nutr Prod France, NRD CA, Res Ctr Anim Nutr & Hlth, St Louis, France
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2013年 / 165卷 / 01期
关键词
Astaxanthin; Cholesterol; Atlantic salmon; Lipoprotein; SERUM-LIPOPROTEINS; LIPID-COMPOSITION; BINDING-PROTEIN; BETA-CAROTENE; FATTY-ACIDS; CANTHAXANTHIN; DENSITY; L; ABSORPTION; METABOLISM;
D O I
10.1016/j.cbpb.2013.02.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of dietary cholesterol on astaxanthin (Ax) absorption and transport in the plasma of Atlantic salmon was investigated. Under controlled conditions, three experimental diets, non-pigmented diet (NPD), NPD with 40 mg Ax kg(-1) , and NPD with 40 mg Ax kg(-1) and 2% cholesterol, were fed to juvenile salmon reared in sea water. After 12 weeks, blood was collected and plasma separated for analysis of plasma Ax and cholesterol content. In addition, plasma samples from each group of fish were fractionated into lipoproteins using a sucrose density gradient and ultracentrifugation. The apolipoprotein components of VLDL, LDL and HDL from each sample fraction were separated using SDS-PAGE. The addition of 2% cholesterol to the Ax-containing diet significantly increased the concentration of Ax and cholesterol in fish plasma. The protein-rich fraction was found to be the major carder of Ax in salmon plasma. Cholesterol supplementation significantly increased Ax in plasma and VLDL as well as increasing plasma cholesterol. The VLDL fraction showed the most significant change in fish fed diet supplemented with cholesterol resulting in higher levels of Ax in this lipoprotein. The results clearly show that dietary cholesterol had a significant effect on the Ax transport process in the blood. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:73 / 81
页数:9
相关论文
共 64 条
[1]   Blood appearance, metabolic transformation and plasma transport proteins of 14C-astaxanthin in Atlantic salmon (Salmo salar L.) [J].
Aas, GH ;
Bjerkeng, B ;
Storebakken, T ;
Ruyter, B .
FISH PHYSIOLOGY AND BIOCHEMISTRY, 1999, 21 (04) :325-334
[2]   Isolation of low density lipoprotein subfraction containing apolipoprotein B-like protein from Japanese eel Anguilla japonica plasma using dextran sulfate cellulose [J].
Ando, S ;
Matsuzaki, M .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1997, 116 (02) :191-196
[3]   BILIRUBIN-BINDING PROTEIN IN THE SERUM OF SPAWNING-MIGRATING CHUM SALMON, ONCORHYNCHUS-KETA - ITS IDENTITY WITH CAROTENOID-CARRYING LIPOPROTEIN [J].
ANDO, S ;
HATANO, M .
FISH PHYSIOLOGY AND BIOCHEMISTRY, 1988, 5 (02) :69-78
[4]   Structure of Fish Serum Albumins [J].
Andreeva, A. M. .
JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY, 2010, 46 (02) :135-144
[5]  
[Anonymous], 1993, NUTR REQ FISH
[6]  
BABIN PJ, 1989, J LIPID RES, V30, P467
[8]   Deposition of astaxanthin in fillets of Atlantic salmon (Salmo salar) fed diets with herring, capelin, sandeel, or Peruvian high PUFA oils [J].
Bjerkeng, B ;
Hatlen, B ;
Wathne, E .
AQUACULTURE, 1999, 180 (3-4) :307-319
[9]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[10]  
Buttle L, 2000, Pigment. International Patent Classification: A23K 1/18, Patent No. 0067591