Selection of biocatalysts for chemical synthesis

被引：70

作者：

Fiet, SV ^{[1
]}

van Beilen, JB ^{[1
]}

Witholt, B ^{[1
]}

机构：

[1] ETH Honggerberg, Inst Biotechnol, CH-8093 Zurich, Switzerland

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2006年 / 103卷 / 06期

关键词：

enzyme selection; reporter genes;

D O I：

10.1073/pnas.0504733102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To determine whether microbial chemosensors can be used to find new or better biocatalysts, we constructed Escherichia coli hosts that recognize the product of a biocatalytic conversion through the transcriptional activator NahR and respond by expression of a lacZ or tetA reporter gene. Equipped with a benzaldehyde dehydrogenase (XyIC from Pseudomonas putida), the lacZ-based host responded to the oxidation of benzaldehyde and 2-hydroxybenzal-dehyde to the corresponding benzoic acids by forming blue colonies, whereas XyIC(-) cells did not. Similarly, the tetA-based host was able to grow under selective conditions only when equipped with XyIC, enabling selection of biocatalytically active cells in inactive populations at frequencies as low as 10(-6).

引用

页码：1693 / 1698

页数：6

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