CYP4 mRNA expression in marine polychaete Perinereis aibuhitensis in response to petroleum hydrocarbon and deltamethrin

被引：36

作者：

Chen, Xue ^{[1
]}

Zhou, Yibing ^{[1
]}

Yang, Dazuo ^{[1
]}

Zhao, Huan ^{[1
]}

Wang, Lili ^{[2
]}

Yuan, Xiutang ^{[2
]}

机构：

[1] Dalian Ocean Univ, Key Lab Marine Bioresources Restorat & Habitat Re, Dalian 116023, Liaoning, Peoples R China

[2] State Ocean Adm, Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China

来源：

MARINE POLLUTION BULLETIN | 2012年 / 64卷 / 09期

基金：

中国国家自然科学基金;

关键词：

CYP4; Perinereis aibuhitensis; Petroleum hydrocarbon; Deltamethrin; Gene expression; CYTOCHROME-P450; GENES; MOLECULAR-CLONING; NEREIS-VIRENS; IDENTIFICATION; TOXICITY; FAMILY; P450; METABOLITES; INDUCTION; SYSTEM;

D O I：

10.1016/j.marpolbul.2012.05.035

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A CYP4 cDNA was cloned and characterized to identify the relationship between persistent organic pollutants and stress response in marine polychaete Perinereis aibuhitensis. The full length of PaCYP4 cDNA is 1857 bp and encodes 481 amino acids. The deduced amino acid sequence showed 73% identity with CYP4BB1 from polychaete Nereis virens and shared high homology to other known CYP4 sequences. The expression level of PaCYP4 under petroleum hydrocarbon (PH) and deltamethrin (DM) exposure was detected using Real-time PCR. PH and combined toxicity treatments elevated the mRNA level of PaCYP4 in a dose- and time-dependent manner. The mRNA transcripts of PaCYP4 increased at the beginning of DM exposure and then eventually decreased, and the expression level of PaCYP4 down-regulated with increasing concentration of DM. CYP4 in P. aibuhitensis plays an important role in the metabolism of petroleum hydrocarbon and organochlorine pesticide. (C) 2012 Published by Elsevier Ltd.

引用

页码：1782 / 1788

页数：7

共 31 条

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