Assessments of injectable alginate particle-embedded fibrin hydrogels for soft tissue reconstruction

被引:35
作者
Hwang, C. M. [1 ]
Ay, B. [1 ]
Kaplan, D. L. [2 ]
Rubin, J. P. [3 ]
Marra, K. G. [3 ]
Atala, A. [1 ]
Yoo, J. J. [1 ]
Lee, S. J. [1 ]
机构
[1] Wake Forest Sch Med, Wake Forest Inst Regenerat Med, Winston Salem, NC 27157 USA
[2] Tufts Univ, Dept Biomed Engn, Medford, MA USA
[3] Univ Pittsburgh, Dept Surg, Div Plast Surg, Pittsburgh, PA USA
关键词
STEM-CELLS; DIFFERENTIATION; SCAFFOLDS; STIFFNESS; SURGERY; GLUE;
D O I
10.1088/1748-6041/8/1/014105
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Soft tissue reconstruction is often needed after massive traumatic damage or cancer removal. In this study, we developed a novel hybrid hydrogel system consisting of alginate particles and a fibrin matrix that could maintain tissue volume long term. Alginate particles were fabricated by mixing 5% alginate with a 20 mM calcium solution. Cells and these alginate particles were then embedded in fibrin (alginate-fibrin) hydrogels using a dual syringe mixer. Cell-hydrogel constructs were evaluated in terms of cell survival and proliferation in the constructs in vitro. The results indicated that cellular viability, spreading and proliferation in the alginate-fibrin hydrogels were significantly higher compared to constructs fabricated with fibrin or alginate only. In vivo explants showed that cells contained within fibrin-only hydrogels did not contribute to neo-tissue formation, and the fibrin was fully degraded within a 12 week period. In the alginate-fibrin system, higher cellularity and vascular ingrowth were observed in vivo. This resulted in neo-tissue formation in the alginate-fibrin hydrogels. These results demonstrate that fibrin may enhance cell proliferation and accelerate the formation of extracellular matrix proteins in the alginate-fibrin system, while the alginate particles could contribute to volume retention. This injectable hybrid system composed of degradable and non-degradable hydrogels may be a preferable approach to the repair of soft tissue defects.
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页数:9
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