A U-Box E3 Ubiquitin Ligase, PUB20, Interacts with the Arabidopsis G-Protein β Subunit, AGB1

被引：16
作者：
Kobayashi, Shio [1 ]
Tsugama, Daisuke [1 ]
Liu, Shenkui [2 ]
Takano, Tetsuo [1 ]
机构：
[1] Univ Tokyo, ANESC, Tokyo, Japan
[2] NE Forestry Univ, ASNESC, Harbin, Peoples R China
来源：
PLOS ONE | 2012年 / 7卷 / 11期
关键词：
HETEROTRIMERIC G-PROTEIN; ABSCISIC-ACID REGULATION; PUCCINELLIA-TENUIFLORA; THALIANA; RICE; EXPRESSION; K+;
D O I：
10.1371/journal.pone.0049207
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
An Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein beta subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with AGB1 in the nuclei and the cytosol. The expression levels of PUB20 and its closest homolog, PUB21 were stable under many conditions. GUS driven by the PUB20 promoter was active in anthers, pollen, premature seeds and receptacles and GUS driven by the PUB21 promoter was active in anthers and funiculi. PUB20 was found to have autoubiquitination activity in vitro.
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页数：6
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