A U-Box E3 Ubiquitin Ligase, PUB20, Interacts with the Arabidopsis G-Protein β Subunit, AGB1

被引：16

作者：

Kobayashi, Shio ^{[1
]}

Tsugama, Daisuke ^{[1
]}

Liu, Shenkui ^{[2
]}

Takano, Tetsuo ^{[1
]}

机构：

[1] Univ Tokyo, ANESC, Tokyo, Japan

[2] NE Forestry Univ, ASNESC, Harbin, Peoples R China

来源：

PLOS ONE | 2012年 / 7卷 / 11期

关键词：

HETEROTRIMERIC G-PROTEIN; ABSCISIC-ACID REGULATION; PUCCINELLIA-TENUIFLORA; THALIANA; RICE; EXPRESSION; K+;

D O I：

10.1371/journal.pone.0049207

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein beta subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with AGB1 in the nuclei and the cytosol. The expression levels of PUB20 and its closest homolog, PUB21 were stable under many conditions. GUS driven by the PUB20 promoter was active in anthers, pollen, premature seeds and receptacles and GUS driven by the PUB21 promoter was active in anthers and funiculi. PUB20 was found to have autoubiquitination activity in vitro.

引用

页数：6

共 21 条

[1] Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein β-subunit AGB1
Anderson, David J.
Botella, Jose R.
[J]. PLANT CELL REPORTS, 2007, 26 (09) : 1469 - 1480
[2] Ectopic Expression of the K+ Channel β Subunits from Puccinellia tenuiflora (KPutB1) and Rice (KOB1) Alters K+ Homeostasis of Yeast and Arabidopsis
Ardie, Sintho Wahyuning
Nishiuchi, Shunsaku
Liu, Shenkui
Takano, Tetsuo
[J]. MOLECULAR BIOTECHNOLOGY, 2011, 48 (01) : 76 - 86
[3] Characterization of two plasma membrane protein 3 genes (PutPMP3) from the alkali grass, Puccinellia tenuiflora, and functional comparison of the rice homologues, OsLti6a/b from rice
Chang-Qing, Zhang
Shunsaku, Nishluchi
Shenkui, Liu
Tetsuo, Takano
[J]. BMB REPORTS, 2008, 41 (06) : 448 - 454
[4] GCR1 can act independently of heterotrimeric G-protein in response to brassinosteroids and gibberellins in Arabidopsis seed germination
Chen, JG
Pandey, S
Huang, JR
Alonso, JM
Ecker, JR
Assmann, SM
Jones, AM
[J]. PLANT PHYSIOLOGY, 2004, 135 (02) : 907 - 915
[5] C-terminal armadillo repeats are essential and sufficient for association of the plant U-box armadillo E3 ubiquitin ligase SAUL1 with the plasma membrane
Drechsel, Gabriele
Bergler, Johannes
Wippel, Kathrin
Sauer, Norbert
Vogelmann, Katja
Hoth, Stefan
[J]. JOURNAL OF EXPERIMENTAL BOTANY, 2011, 62 (02) : 775 - 785
[6] Abscisic acid regulation of guard-cell K+ and anion channels in Gβ- and RGS-deficient Arabidopsis lines
Fan, Liu-Min
Zhang, Wei
Chen, Jin-Gui
Taylor, J. Philip
Jones, Alan M.
Assmann, Sarah M.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (24) : 8476 - 8481
[7] Two immediate-early pathogen-responsive of the AtCMPG gene family in Arabidopsis thaliana and the W-box-containing elicitor-response element of AtCMPG1
Heise, A
Lippok, B
Kirsch, C
Hahlbrock, K
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (13) : 9049 - 9054
[8] A mutant Arabidopsis heterotrimeric G-protein β subunit affects leaf, flower, and fruit development
Lease, KA
Wen, JQ
Li, J
Doke, JT
Liscum, E
Walker, JC
[J]. PLANT CELL, 2001, 13 (12) : 2631 - 2641
[9] Characterization of Arabidopsis and rice DWD proteins and their roles as substrate receptors for CUL4-RING E3 ubiquitin ligases
Lee, Jae-Hoon
Terzaghi, William
Gusmaroli, Giuliana
Charron, Jean-Benoit F.
Yoon, Hye-Jin
Chen, Haodong
He, Yizhou Joseph
Xiong, Yue
Deng, Xing Wang
[J]. PLANT CELL, 2008, 20 (01) : 152 - 167
[10] Completing the heterotrimer:: Isolation and characterization of an Arabidopsis thaliana G protein γ-subunit cDNA
Mason, MG
Botella, JR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (26) : 14784 - 14788

← 1 2 3 →