The Development of Highly Sensitive and Selective Immunosensor Based on Antibody Immobilized ZnO Nanorods for the Detection of D-Dimer

被引：27
作者：
Ibupoto, Zafar H. [1 ]
Mitrou, Nikolaos [2 ]
Nikoleli, Georgia-Paraskevi [3 ]
Nikolelis, Dimitrios P. [2 ]
Willander, Magnus [1 ]
Psaroudakis, Nikolas [4 ]
机构：
[1] Linkoping Univ, Dept Sci & Technol, Phys Elect & Nanotechnol Div, SE-60174 Norrkoping, Sweden
[2] Univ Athens, Dept Chem, Environm Chem Lab, GR-15771 Athens, Greece
[3] Natl Tech Univ Athens, Sch Chem Engn, Dept 1, Lab Inorgan & Analyt Chem, Athens 15780, Greece
[4] Univ Athens, Dept Chem, Inorgan Chem Lab, GR-15771 Athens, Greece
来源：
ELECTROANALYSIS | 2014年 / 26卷 / 02期
关键词：
ZnO nanorods; D-Dimer; Immunosensors; Potentiometric technique; DEEP-VEIN THROMBOSIS; STABILIZED LIPID-MEMBRANE; PULMONARY-EMBOLISM; BIOSENSORS; THROMBOEMBOLISM; IMMUNOASSAY; NANOTUBES; ENZYME;
D O I：
10.1002/elan.201300580
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
ZnO nanorods were grown on gold coated glass substrate by low temperature aqueous chemical growth method. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques were used for the characterization of ZnO nanorods. ZnO nanorods are highly dense, uniform, well aligned and perpendicular-oriented to the substrate. ZnO nanorods exhibited good crystal quality. The well aligned ZnO nanorods were potentially used for the development of selective and sensitive immunosensor for the detection of D-dimer by immobilizing antibody on stabilized lipid films. The ZnO nanorods based immunosensor responded to a wide range of D-dimer concentrations with fast response time of ca. 20s.
引用
收藏
页码：292 / 298
页数：7
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