The Development of Highly Sensitive and Selective Immunosensor Based on Antibody Immobilized ZnO Nanorods for the Detection of D-Dimer

被引:27
作者
Ibupoto, Zafar H. [1 ]
Mitrou, Nikolaos [2 ]
Nikoleli, Georgia-Paraskevi [3 ]
Nikolelis, Dimitrios P. [2 ]
Willander, Magnus [1 ]
Psaroudakis, Nikolas [4 ]
机构
[1] Linkoping Univ, Dept Sci & Technol, Phys Elect & Nanotechnol Div, SE-60174 Norrkoping, Sweden
[2] Univ Athens, Dept Chem, Environm Chem Lab, GR-15771 Athens, Greece
[3] Natl Tech Univ Athens, Sch Chem Engn, Dept 1, Lab Inorgan & Analyt Chem, Athens 15780, Greece
[4] Univ Athens, Dept Chem, Inorgan Chem Lab, GR-15771 Athens, Greece
关键词
ZnO nanorods; D-Dimer; Immunosensors; Potentiometric technique; DEEP-VEIN THROMBOSIS; STABILIZED LIPID-MEMBRANE; PULMONARY-EMBOLISM; BIOSENSORS; THROMBOEMBOLISM; IMMUNOASSAY; NANOTUBES; ENZYME;
D O I
10.1002/elan.201300580
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
ZnO nanorods were grown on gold coated glass substrate by low temperature aqueous chemical growth method. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques were used for the characterization of ZnO nanorods. ZnO nanorods are highly dense, uniform, well aligned and perpendicular-oriented to the substrate. ZnO nanorods exhibited good crystal quality. The well aligned ZnO nanorods were potentially used for the development of selective and sensitive immunosensor for the detection of D-dimer by immobilizing antibody on stabilized lipid films. The ZnO nanorods based immunosensor responded to a wide range of D-dimer concentrations with fast response time of ca. 20s.
引用
收藏
页码:292 / 298
页数:7
相关论文
共 39 条
  • [1] Impedimetric immunosensor based on SWCNT-COOH modified gold microelectrodes for label-free detection of deep venous thrombosis biomarker
    Bourigua, S.
    Hnaien, M.
    Bessueille, F.
    Lagarde, F.
    Dzyadevych, S.
    Maaref, A.
    Bausells, J.
    Errachid, A.
    Renault, N. Jaffrezic
    [J]. BIOSENSORS & BIOELECTRONICS, 2010, 26 (04) : 1278 - 1282
  • [2] REGENERABLE FIBER-OPTIC-BASED IMMUNOSENSOR
    BRIGHT, FV
    BETTS, TA
    LITWILER, KS
    [J]. ANALYTICAL CHEMISTRY, 1990, 62 (10) : 1065 - 1069
  • [3] Multiwalled Carbon Nanotubes Modified by NTA-Copper Complex for Label-Free Electrochemical Immunosensor Detection
    Chebil, S.
    Macauley, N.
    Hianik, T.
    Korri-Youssoufi, H.
    [J]. ELECTROANALYSIS, 2013, 25 (03) : 636 - 643
  • [4] Electrochemical detection of D-dimer as deep vein thrombosis marker using single-chain D-dimer antibody immobilized on functionalized polypyrrole
    Chebil, S.
    Hafaiedh, I.
    Sauriat-Dorizon, H.
    Jaffrezic-Renault, N.
    Errachid, A.
    Ali, Z.
    Korri-Youssoufi, H.
    [J]. BIOSENSORS & BIOELECTRONICS, 2010, 26 (02) : 736 - 742
  • [5] Chu Arthur J., 2004, Current Vascular Pharmacology, V2, P199, DOI 10.2174/1570161043385673
  • [6] Deep vein thrombosis and pulmonary embolism in two cohorts: The longitudinal investigation of thromboembolism etiology
    Cushman, M
    Tsai, AW
    White, RH
    Heckbert, SR
    Rosamond, WD
    Enright, P
    Folsom, AR
    [J]. AMERICAN JOURNAL OF MEDICINE, 2004, 117 (01) : 19 - 25
  • [7] Novel nanostructures of functional oxides synthesized by thermal evaporation
    Dai, ZR
    Pan, ZW
    Wang, ZL
    [J]. ADVANCED FUNCTIONAL MATERIALS, 2003, 13 (01) : 9 - 24
  • [8] Amperometric Magnetoimmunosensors for Direct Determination of D-Dimer in Human Serum
    Gamella, Maria
    Campuzano, Susana
    Conzuelo, Felipe
    Julio Reviejo, A.
    Pingarron, Jose M.
    [J]. ELECTROANALYSIS, 2012, 24 (12) : 2235 - 2243
  • [9] Validation of a new D-dimer microparticle enzyme immunoassay (AxSYM D-Dimer) in patients with suspected pulmonary embolism (PE)
    Ghanima, W.
    Sandset, P. M.
    [J]. THROMBOSIS RESEARCH, 2007, 120 (04) : 471 - 476
  • [10] CLINICAL-ASSESSMENT OF STROKE
    GOLDSTEIN, LB
    MATCHAR, DB
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1994, 271 (14): : 1114 - 1120