The Development of Highly Sensitive and Selective Immunosensor Based on Antibody Immobilized ZnO Nanorods for the Detection of D-Dimer

被引：27

作者：

Ibupoto, Zafar H. ^{[1
]}

Mitrou, Nikolaos ^{[2
]}

Nikoleli, Georgia-Paraskevi ^{[3
]}

Nikolelis, Dimitrios P. ^{[2
]}

Willander, Magnus ^{[1
]}

Psaroudakis, Nikolas ^{[4
]}

机构：

[1] Linkoping Univ, Dept Sci & Technol, Phys Elect & Nanotechnol Div, SE-60174 Norrkoping, Sweden

[2] Univ Athens, Dept Chem, Environm Chem Lab, GR-15771 Athens, Greece

[3] Natl Tech Univ Athens, Sch Chem Engn, Dept 1, Lab Inorgan & Analyt Chem, Athens 15780, Greece

[4] Univ Athens, Dept Chem, Inorgan Chem Lab, GR-15771 Athens, Greece

来源：

ELECTROANALYSIS | 2014年 / 26卷 / 02期

关键词：

ZnO nanorods; D-Dimer; Immunosensors; Potentiometric technique; DEEP-VEIN THROMBOSIS; STABILIZED LIPID-MEMBRANE; PULMONARY-EMBOLISM; BIOSENSORS; THROMBOEMBOLISM; IMMUNOASSAY; NANOTUBES; ENZYME;

D O I：

10.1002/elan.201300580

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

ZnO nanorods were grown on gold coated glass substrate by low temperature aqueous chemical growth method. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques were used for the characterization of ZnO nanorods. ZnO nanorods are highly dense, uniform, well aligned and perpendicular-oriented to the substrate. ZnO nanorods exhibited good crystal quality. The well aligned ZnO nanorods were potentially used for the development of selective and sensitive immunosensor for the detection of D-dimer by immobilizing antibody on stabilized lipid films. The ZnO nanorods based immunosensor responded to a wide range of D-dimer concentrations with fast response time of ca. 20s.

引用

页码：292 / 298

页数：7

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