Application of electrochemical properties of ordered mesoporous carbon to the determination of glutathione and cysteine

被引：120

作者：

Ndamanisha, Jean Chrysostome ^{[1
]}

Bai, Jing ^{[1
]}

Qj, Bin ^{[1
]}

Guo, Liping ^{[1
]}

机构：

[1] NE Normal Univ, Fac Chem, Changchun 130024, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 386卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Ordered mesoporous carbon; Electrocatalytic; Glutathione; Cysteine; Determination; SELECTIVE DETERMINATION; AMPEROMETRIC DETECTION; SENSITIVE DETECTION; PYROLYTIC-GRAPHITE; ELECTRON-TRANSFER; GOLD ELECTRODE; L-CYSTINE; OXIDATION; NANOTUBES; ELECTROCATALYSIS;

D O I：

10.1016/j.ab.2008.11.041

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, the electrochemical activity of ordered mesoporous carbon (OMC) was investigated and applied to the determination of glutathione (GSH) and cysteine (CySH). It has been demonstrated that the ordered mesostructure of OMC has an important role in the electrocatalytic activity towards thiols, and the destruction Of this Structure results in the decrease of such properties. The electrochemical behavior of GSH at an OMC electrode was also investigated. The results showed that the process of oxidation of GSH at the OMC electrode is differs from that of CySH at the same electrode by the peak at 0.47 V associated with CySH. This difference helped to reduce the interference of GSH during the determination of CySH in the presence of GSH. A sensor for the two thiols was developed with acceptable sensitivity and detection limits in a large determination range. These results obtained in the physiological medium and in the physiological levels of GSH and CySH, Suggest that OMC is a promising material in the detection of thiols in biologically relevant experimental conditions (in terms of pH). (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：79 / 84

页数：6

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