Generation of new Notch2 mutant alleles

被引:82
作者
McCright, B [1 ]
Lozier, J [1 ]
Gridley, T [1 ]
机构
[1] Jackson Lab, Bar Harbor, ME 04609 USA
关键词
Notch signaling; conditional null allele; Cre-IoxP; gene targeting;
D O I
10.1002/gene.20181
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Notch signaling pathway is an evolutionarily conserved intercellular signaling mechanism, and mutations in its components disrupt embryonic development in many organisms and cause inherited diseases in humans. We previously described construction and analysis of a hypomorphic allele of the Notch2 gene. Homozygosity for this allele leads to embryonic and perinatal lethality due to cardiovascular and kidney defects. We report here novel Notch2 mutant alleles generated by gene targeting in embryonic stem cells, including a conditional null allele in which exon 3 of the Notch2 gene is flanked by loxP sequences. These new Notch2 mutant alleles expand the set of tools available for studying the myriad roles of the Notch pathway during mammalian development and will enable analysis of Notch2 function at additional stages of embryogenesis and in adult mice. genesis 44:29-33, 2006. (c) 2006 Wiley-Liss, Inc.
引用
收藏
页码:29 / 33
页数:5
相关论文
共 17 条
[1]   PPARγ is required for placental, cardiac, and adipose tissue development [J].
Barak, Y ;
Nelson, MC ;
Ong, ES ;
Jones, YZ ;
Ruiz-Lozano, P ;
Chien, KR ;
Koder, A ;
Evans, RM .
MOLECULAR CELL, 1999, 4 (04) :585-595
[2]  
Farley FW, 2000, GENESIS, V28, P106, DOI 10.1002/1526-968X(200011/12)28:3/4<106::AID-GENE30>3.0.CO
[3]  
2-T
[4]   Notch signaling and inherited disease syndromes [J].
Gridley, T .
HUMAN MOLECULAR GENETICS, 2003, 12 :R9-R13
[5]  
Hamada Y, 1999, DEVELOPMENT, V126, P3415
[6]   PPARγ mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance [J].
Kubota, N ;
Terauchi, Y ;
Miki, H ;
Tamemoto, H ;
Yamauchi, T ;
Komeda, K ;
Satoh, S ;
Nakano, R ;
Ishii, C ;
Sugiyama, T ;
Eto, K ;
Tsubamoto, Y ;
Okuno, A ;
Murakami, K ;
Sekihara, H ;
Hasegawa, G ;
Naito, M ;
Toyoshima, Y ;
Tanaka, S ;
Shiota, K ;
Kitamura, T ;
Fujita, T ;
Ezaki, O ;
Aizawa, S ;
Nagai, R ;
Tobe, K ;
Kimura, S ;
Kadowaki, T .
MOLECULAR CELL, 1999, 4 (04) :597-609
[7]   Notch signaling: control of cell communication and cell fate [J].
Lai, EC .
DEVELOPMENT, 2004, 131 (05) :965-973
[8]   The roles of receptor and ligand endocytosis in regulating Notch signaling [J].
Le Borgne, R ;
Bardin, A ;
Schweisguth, F .
DEVELOPMENT, 2005, 132 (08) :1751-1762
[9]  
McCright B, 2001, DEVELOPMENT, V128, P491
[10]  
Nakayama H, 1997, DEV GENET, V21, P21, DOI 10.1002/(SICI)1520-6408(1997)21:1<21::AID-DVG3>3.3.CO