The Cdc48 Protein and Its Cofactor Vms1 Are Involved in Cdc13 Protein Degradation

被引：13

作者：

Baek, Guem Hee ^{[1
]}

Cheng, Haili ^{[1
]}

Kim, Ikjin ^{[1
]}

Rao, Hai ^{[1
]}

机构：

[1] Univ Texas Hlth Sci Ctr San Antonio, Inst Biotechnol, Dept Mol Med, San Antonio, TX 78245 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2012年 / 287卷 / 32期

基金：

美国国家卫生研究院;

关键词：

SACCHAROMYCES-CEREVISIAE; UBIQUITIN LIGASE; COMPLEXES; YEAST; UFD2; TURNOVER; ATPASE; SYSTEM;

D O I：

10.1074/jbc.M112.351825

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vms1 is a newly identified Cdc48-binding protein. The biological function of Vms1 remains obscure. Here, we show that both Cdc48 and Vms1, but not Cdc48 cofactors Ufd1 and Ufd2, are crucial for the degradation of Cdc13, a telomere regulator. Interestingly, both autophagy and the proteasome are involved in Cdc13 turnover. Toxicity associated with accumulation of large amounts of Cdc13 in vms1 Delta or autophagy mutants underscores the significance of the proteolytic regulation of Cdc13. Because few ubiquitylated yeast proteins are known to be degraded by autophagy under non-stress conditions, the identification of Cdc13 as a target of autophagy provides a valuable tool to unravel the mechanism of autophagy-mediated selective protein degradation.

引用

页码：26788 / 26795

页数：8

共 24 条

[1] VCP Associated Inclusion Body Myopathy and Paget Disease of Bone Knock-In Mouse Model Exhibits Tissue Pathology Typical of Human Disease [J].

Badadani, Mallikarjun ;

Nalbandian, Angele ;

Watts, Giles D. ;

Vesa, Jouni ;

Kitazawa, Masashi ;

Su, Hailing ;

Tanaja, Jasmin ;

Dec, Eric ;

Wallace, Douglas C. ;

Mukherjee, Jogeshwar ;

Caiozzo, Vincent ;

Warman, Matthew ;

Kimonis, Virginia E. .

PLOS ONE, 2010, 5 (10)

[2] The Cdc48 ATPase modulates the interaction between two proteolytic factors Ufd2 and Rad23 [J].

Baek, Guem Hee ;

Kim, Ikjin ;

Rao, Hai .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (33) :13558-13563

[3]

Buchberger A, 2010, SUBCELL BIOCHEM, V54, P17, DOI 10.1007/978-1-4419-6676-6_2

[4] Functional organization of the yeast proteome by systematic analysis of protein complexes [J].

Gavin, AC ;

Bösche, M ;

Krause, R ;

Grandi, P ;

Marzioch, M ;

Bauer, A ;

Schultz, J ;

Rick, JM ;

Michon, AM ;

Cruciat, CM ;

Remor, M ;

Höfert, C ;

Schelder, M ;

Brajenovic, M ;

Ruffner, H ;

Merino, A ;

Klein, K ;

Hudak, M ;

Dickson, D ;

Rudi, T ;

Gnau, V ;

Bauch, A ;

Bastuck, S ;

Huhse, B ;

Leutwein, C ;

Heurtier, MA ;

Copley, RR ;

Edelmann, A ;

Querfurth, E ;

Rybin, V ;

Drewes, G ;

Raida, M ;

Bouwmeester, T ;

Bork, P ;

Seraphin, B ;

Kuster, B ;

Neubauer, G ;

Superti-Furga, G .

NATURE, 2002, 415 (6868) :141-147

[5] A Stress-Responsive System for Mitochondrial Protein Degradation [J].

Heo, Jin-Mi ;

Livnat-Levanon, Nurit ;

Taylor, Eric B. ;

Jones, Kevin T. ;

Dephoure, Noah ;

Ring, Julia ;

Xie, Jianxin ;

Brodsky, Jeffrey L. ;

Madeo, Frank ;

Gygi, Steven P. ;

Ashrafi, Kaveh ;

Glickman, Michael H. ;

Rutter, Jared .

MOLECULAR CELL, 2010, 40 (03) :465-480

[6] Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry [J].

Ho, Y ;

Gruhler, A ;

Heilbut, A ;

Bader, GD ;

Moore, L ;

Adams, SL ;

Millar, A ;

Taylor, P ;

Bennett, K ;

Boutilier, K ;

Yang, LY ;

Wolting, C ;

Donaldson, I ;

Schandorff, S ;

Shewnarane, J ;

Vo, M ;

Taggart, J ;

Goudreault, M ;

Muskat, B ;

Alfarano, C ;

Dewar, D ;

Lin, Z ;

Michalickova, K ;

Willems, AR ;

Sassi, H ;

Nielsen, PA ;

Rasmussen, KJ ;

Andersen, JR ;

Johansen, LE ;

Hansen, LH ;

Jespersen, H ;

Podtelejnikov, A ;

Nielsen, E ;

Crawford, J ;

Poulsen, V ;

Sorensen, BD ;

Matthiesen, J ;

Hendrickson, RC ;

Gleeson, F ;

Pawson, T ;

Moran, MF ;

Durocher, D ;

Mann, M ;

Hogue, CWV ;

Figeys, D ;

Tyers, M .

NATURE, 2002, 415 (6868) :180-183

[7] The Png1-Rad23 complex regulates glycoprotein turnover [J].

Kim, I ;

Ahn, J ;

Liu, C ;

Tanabe, K ;

Apodaca, J ;

Suzuki, T ;

Rao, H .

JOURNAL OF CELL BIOLOGY, 2006, 172 (02) :211-219

[8] Ubiquitin networks in cancer [J].

Kirkin, Vladimir ;

Dikic, Ivan .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2011, 21 (01) :21-28

[9] Mature ribosomes are selectively degraded upon starvation by an autophagy pathway requiring the Ubp3p/Bre5p ubiquitin protease [J].

Kraft, Claudine ;

Deplazes, Anna ;

Sohrmann, Marc ;

Peter, Matthias .

NATURE CELL BIOLOGY, 2008, 10 (05) :602-610

[10] Global landscape of protein complexes in the yeast Saccharomyces cerevisiae [J].

Krogan, NJ ;

Cagney, G ;

Yu, HY ;

Zhong, GQ ;

Guo, XH ;

Ignatchenko, A ;

Li, J ;

Pu, SY ;

Datta, N ;

Tikuisis, AP ;

Punna, T ;

Peregrín-Alvarez, JM ;

Shales, M ;

Zhang, X ;

Davey, M ;

Robinson, MD ;

Paccanaro, A ;

Bray, JE ;

Sheung, A ;

Beattie, B ;

Richards, DP ;

Canadien, V ;

Lalev, A ;

Mena, F ;

Wong, P ;

Starostine, A ;

Canete, MM ;

Vlasblom, J ;

Wu, S ;

Orsi, C ;

Collins, SR ;

Chandran, S ;

Haw, R ;

Rilstone, JJ ;

Gandi, K ;

Thompson, NJ ;

Musso, G ;

St Onge, P ;

Ghanny, S ;

Lam, MHY ;

Butland, G ;

Altaf-Ui, AM ;

Kanaya, S ;

Shilatifard, A ;

O'Shea, E ;

Weissman, JS ;

Ingles, CJ ;

Hughes, TR ;

Parkinson, J ;

Gerstein, M .

NATURE, 2006, 440 (7084) :637-643

← 1 2 3 →