EXTRACTIVE SPECTROPHOTOMETRIC ANALYTICAL METHODS FOR THE DETERMINATION OF PIOGLITAZONE HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORM AND ITS COMPARISON WITH RP-HPLC AND TLC DENSITOMETRIC METHODS

The present study aimed at the development and validation of a first-derivative ultraviolet (UV) spectrophotometric method for the estimation of pioglitazone hydrochloride in bulk and pharmaceutical dosage form which is based on the measurement of absorption maxima at 385 urn. The linearity was observed in the range from 5 to 30 mu g/ml. A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of pioglitazone hydrochloride in tablet dosage form. Isocratic elution at a flow rate of 1.0 ml/min was employed on a symmetry 25 cm x 4.6 mm i.d., 5-mu m particle, Phenomenex Luna C18 column at ambient temperature. The mobile phase consisted of acetonitrile:methanol 80:20 (v/v). The UV detection wavelength was 385 nm and 20-mu l sample was injected. The retention time for pioglitazone hydrochloride was 3.685 min. High performance thin layer chromatographic method has been developed and validated for the estimation of pioglitazone hydrochloride in tablet dosage forms. The method employed TLC aluminium plates pre-coated with silica gel 60 F 254 as a stationary phase. The mobile phase used was a mixture of glacial acetic acid: methanol: carbon tetrachloride (4:2:4 v/v/v). The detection of spot was carried out at 289 nm. The calibration curve was found to be linear between 100 and 700 ng ml(-1) with regression coefficient of 0.9998.