Isolation and characterization of lectins from stem and leaves of Korean mistletoe (Viscum album var. coloratum) by affinity chromatography

被引：16

作者：

Park, WB

Han, SK

Lee, MH

Han, KH

机构：

[1] Department of Chemistry, Seoul Women's University

来源：

ARCHIVES OF PHARMACAL RESEARCH | 1997年 / 20卷 / 04期

关键词：

hemagglutinating activity; lectin; Korean mistletoe; mitogenic; affinity chromatography;

D O I：

10.1007/BF02976191

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We attempted to isolate and characterize the lectins from stem and leaves of Korean mistletoe (Viscum album var. coloratum) by affinity chromatography. Lectin I was isolated only from stem. Lectin II was not isolated from Korean mistletoe, whereas lectin III was isolated from the stem and leaves. The hemagglutinating activity of lectin I was 16HU and inhibited by D-galactose, lactose, and N-acetyl-D-galactosamine. The lectin I has molecular weight of 60,000D being composed of two basic subunits with molecular weights of 32,000D and 28,000D which are linked by a disufide bond. The lectin III from stem has molecular weight of 66,000D being two basic subunits which have molecular weights of 34,000D and 29,000D and are linked by a disufide bond. The activity of lectin I was stable at the pH range of 4.00 similar to 8.50 and at a wide range of temperature (0 similar to 42 degrees C). The lectin I showed more potent mitogenic activity to murine lymphocytes than concanavalin A.

引用

页码：306 / 312

页数：7