Detection and determination of some phenolic and cinnamic acids in plant extracts

被引:4
作者
Sipkina, N. Yu. [1 ]
Skorik, Yu. A. [1 ,2 ]
机构
[1] St Petersburg State Chem Pharmaceut Acad, St Petersburg 197022, Russia
[2] Russian Acad Sci, Inst High Mol Cpds, St Petersburg 199004, Russia
关键词
phenolic acids; cinnamic acids; HPLC; plant extracts;
D O I
10.1134/S1061934815110143
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Chromatographic conditions were optimized and performance characteristics were evaluated for a procedure for the detection and determination of 12 phenolic and cinnamic acids in plant extracts by HPLC with spectrophotometric detection. Chromatographic separation was performed in the gradient elution mode, using an aqueous solution of trifluoroacetic acid and acetonitrile as a mobile phase. Sensitivity was increased by performing spectrophotometric detection at the absorbance maxima of individual acids. The procedure was used for the detection and determination of phenolic content in dried extracts from Astragalus dolichophyllus and Astragalus brachylobus.
引用
收藏
页码:1406 / 1411
页数:6
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