In vitro effects of rebaudioside A, stevioside and steviol on porcine cytochrome p450 expression and activity

被引：11

作者：

Thogersen, Rebekka ^{[1
]}

Petrat-Melin, Bjorn ^{[1
]}

Zamaratskaia, Galia ^{[2
,3
]}

Grevsen, Kai ^{[1
]}

Young, Jette Feveile ^{[1
]}

Rasmussen, Martin Kroyer ^{[1
]}

机构：

[1] Aarhus Univ, Dept Food Sci, Blichers Alle 20, DK-8830 Tjele, Denmark

[2] Swedish Univ Agr Sci, Dept Mol Sci, POB 7015, SE-75007 Uppsala, Sweden

[3] Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Zatisi 728-2, Vodnany 38925, Czech Republic

来源：

FOOD CHEMISTRY | 2018年 / 258卷

关键词：

IPEC-J2; Primary hepatocytes; Food-drug interaction; Natural sweeteners; Stevia rebaudiana; CELL-LINE; HEPATIC MICROSOMES; NATURAL SWEETENER; NUCLEAR RECEPTORS; DRUG-METABOLISM; MESSENGER-RNA; CACO-2; INDUCTION; ENZYMES; PIGS;

D O I：

10.1016/j.foodchem.2018.03.055

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The physiological effects of the Stevia-derived compounds, rebaudioside A, stevioside and steviol have been the focus of several studies due to their use as sweeteners in food. Despite that, little is known about their potential food-drug interactions. In the present study, IPEC-J2 cells and primary hepatocytes were used to investigate the effect of rebaudioside A, stevioside and steviol on cytochrome p450 (CYP) mRNA expression. Moreover, hepatic microsomes were used to investigate direct interactions between the compounds and specific CYP activity. In IPEC-J2 no changes in mRNA expression of CYP1A1 or CYP3A29 were observed with the Stevia-derived compounds. In primary hepatocytes all three tested compounds induced a significant increase in CYP3A29 expression. The tested compounds had no direct effect on specific CYP activity. In conclusion, rebaudioside A, stevioside and steviol induce only minor or no changes to the CYP expression and activity, and are not likely to cause food-drug interactions.

引用

页码：245 / 253

页数：9

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