Co-culture with endothelial progenitor cells promotes survival, migration, and differentiation of osteoclast precursors

被引：20

作者：

Pang, Hao ^{[1
]}

Wu, Xue-Hui ^{[1
]}

Fu, Sheng-Long ^{[1
]}

Luo, Fei ^{[1
]}

Zhang, Ze-Hua ^{[1
]}

Hou, Tian-Yong ^{[1
]}

Li, Zhi-Qiang ^{[1
]}

Chang, Zheng-Qi ^{[1
]}

Yu, Bo ^{[1
]}

Xu, Jian-Zhong ^{[1
]}

机构：

[1] Third Mil Med Univ, Dept Orthopaed, Southwest Hosp, Chongqing, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 430卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Endothelial progenitor cells; Co-culture; RAW; 264.7; cells; Survival; Migration; Differentiation; GROWTH-FACTOR-BETA; CHEMOKINE RECEPTOR CXCR4; BONE; RECRUITMENT; PATHWAYS; RANKL; VEGF;

D O I：

10.1016/j.bbrc.2012.11.081

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we report the effect of endothelial progenitor cells (EPCs) on the biological behavior of osteoclast precursors in vitro by establishing an indirect co-culture system of mice EPCs and RAW 264.7 monocyte cells. Results show that the survival, migration, and differentiation of osteoclast precursors were greatly enhanced when co-cultured with EPCs. These phenotypic changes coincide with the upregulation of multiple genes affected cell behavior, including phospho-VEGFR-2, CXCR4, phospho-Smad2/3, phospho-Akt, phospho-ERK1, and phospho-p38 MAPK. The results collectively suggest that EPCs could modulate the survival, migration, and differentiation potential of osteoclast precursors, thus providing new insights in understanding of correlation between angiogenesis and bone homeostasis. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：729 / 734

页数：6

共 25 条

[1] The molecular understanding of osteoclast differentiation
Asagiri, Masataka
Takayanagi, Hiroshi
[J]. BONE, 2007, 40 (02) : 251 - 264
[2] Osteoclast differentiation and activation
Boyle, WJ
Simonet, WS
Lacey, DL
[J]. NATURE, 2003, 423 (6937) : 337 - 342
[3] Burlacu A., 2012, Stem Cells Dev
[4] ERK implication in cell cycle regulation
Chambard, Jean-Claude
Lefloch, Renaud
Pouyssegur, Jacques
Lenormand, Philippe
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2007, 1773 (08): : 1299 - 1310
[5] Remodeling and vascular spaces in bone
Eriksen, Erik Fink
Eghbali-Fatourechi, Guiti Z.
Khosla, Sundeep
[J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2007, 22 (01) : 1 - 6
[6] Geuze RE, 2009, TISSUE ENG PT A, V15, P3669, DOI [10.1089/ten.tea.2009.0289, 10.1089/ten.TEA.2009.0289]
[7] TGF-β coordinately activates TAK1/MEK/AKT/NFkB and SMAD pathways to promote osteoclast survival
Gingery, Anne
Bradley, Elizabeth W.
Pederson, Larry
Ruan, Ming
Horwood, Nikki J.
Oursler, Merry Jo
[J]. EXPERIMENTAL CELL RESEARCH, 2008, 314 (15) : 2725 - 2738
[8] Oral Administration of Blueberry Inhibits Angiogenic Tumor Growth and Enhances Survival of Mice with Endothelial Cell Neoplasm
Gordillo, Gayle
Fang, Huiqing
Khanna, Savita
Harper, Justin
Phillips, Gary
Sen, Chandan K.
[J]. ANTIOXIDANTS & REDOX SIGNALING, 2009, 11 (01) : 47 - 58
[9] RANKL-independent human osteoclast formation with APRIL, BAFF, NGF, IGF I and IGF II
Hemingway, F.
Taylor, R.
Knowles, H. J.
Athanasou, N. A.
[J]. BONE, 2011, 48 (04) : 938 - 944
[10] Osteoclast differentiation requires TAK1 and MKK6 for NFATc1 induction and NF-κB transactivation by RANKL
Huang, H.
Ryu, J.
Ha, J.
Chang, E. -J.
Kim, H. J.
Kim, H. -M.
Kitamura, T.
Lee, Z. H.
Kim, H-H
[J]. CELL DEATH AND DIFFERENTIATION, 2006, 13 (11) : 1879 - 1891

← 1 2 3 →