Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium

被引：176
作者：
Wang, BQ
Li, B
Wang, ZX
Xu, GB
Wang, Q
Dong, SJ [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, Electroanalyt Chem Lab, Changchun 130022, Peoples R China
[2] Chinese Acad Sci, Changchun Inst Appl Chem, Natl Anal & Res Ctr Electrochem & Spect, Changchun 130022, Peoples R China
来源：
ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 10期
关键词：
D O I：
10.1021/ac980940q
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An acid-stable soybean-peroxidase biosensor was devel oped by immobilizing the enzyme in a sol-gel thin film. Methylene blue was used as a mediator because of its high electron-transfer efficiency. The sol-gel thin film and enzyme membrane were characterized by FT-IR, and the effects of pH, operating potential, and temperature were explored for optimum analytical performance by using the amperometric method. The H2O2 sensor exhibited a fast response (5 s), high sensitivity (27.5 mu A/mM), as well as good thermostability and long-term stability. In addition, the performance of the biosensor was investigated using flow-injection analysis (FIA).
引用
收藏
页码：1935 / 1939
页数：5
相关论文
共 34 条
  • [1] Bellamy L J, 1975, INFRARED SPECTRA COM, P374
  • [2] Stability of oxidases immobilized in silica gels
    Chen, Q
    Kenausis, GL
    Heller, A
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (19) : 4582 - 4585
  • [3] ELECTROCATALYTIC OXIDATION OF REDUCED NICOTINAMIDE COENZYMES AT ORGANIC DYE-MODIFIED ELECTRODES
    CHI, Q
    DONG, S
    [J]. ELECTROANALYSIS, 1995, 7 (02) : 147 - 153
  • [4] ELECTROCATALYTIC OXIDATION OF REDUCED NICOTINAMIDE COENZYMES AT METHYLENE GREEN-MODIFIED ELECTRODES AND FABRICATION OF AMPEROMETRIC ALCOHOL BIOSENSORS
    CHI, QJ
    DONG, SJ
    [J]. ANALYTICA CHIMICA ACTA, 1994, 285 (1-2) : 125 - 133
  • [5] CHEMICALLY IMMOBILIZED ENZYME ELECTRODES FOR HYDROGEN-PEROXIDE DETERMINATION
    COSGROVE, M
    MOODY, GJ
    THOMAS, JDR
    [J]. ANALYST, 1988, 113 (12) : 1811 - 1815
  • [6] SOL-GEL ENCAPSULATION METHODS FOR BIOSENSORS
    DAVE, BC
    DUNN, B
    VALENTINE, JS
    ZINK, JI
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (22) : A1120 - A1127
  • [7] FTIR STUDIES OF GEL TO GLASS CONVERSION IN TEOS FUMED SILICA-DERIVED GELS
    DE, G
    KUNDU, D
    KARMAKAR, B
    GANGULI, D
    [J]. JOURNAL OF NON-CRYSTALLINE SOLIDS, 1993, 155 (03) : 253 - 258
  • [8] ORGANIC-PHASE ENZYME ELECTRODE OPERATED IN WATER-FREE SOLVENTS
    DONG, SJ
    GUO, YZ
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (22) : 3895 - 3899
  • [9] GIBSON TD, 1992, ACS S SER, V487, pCH5
  • [10] Encapsulation of biologicals within silicate, siloxane, and hybrid sol-gel polymers: An efficient and generic approach
    Gill, I
    Ballesteros, A
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (34) : 8587 - 8598
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