Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide

被引：88

作者：

Hazim, Roni A. ^{[1
,2
]}

Volland, Stefanie ^{[1
,2
]}

Yen, Alice ^{[1
,2
]}

Burgess, Barry L. ^{[1
,2
]}

Williams, David S. ^{[1
,2
,3
,4
,5
]}

机构：

[1] Univ Calif Los Angeles, Dept Ophthalmol, Los Angeles, CA USA

[2] Univ Calif Los Angeles, Stein Eye Inst, Los Angeles, CA USA

[3] Univ Calif Los Angeles, Dept Neurol, David Geffen Sch Med, Los Angeles, CA 90024 USA

[4] Univ Calif Los Angeles, Mol Biol Inst, Los Angeles, CA 90024 USA

[5] Univ Calif Los Angeles, Brain Res Inst, Los Angeles, CA 90024 USA

来源：

EXPERIMENTAL EYE RESEARCH | 2019年 / 179卷

基金：

美国国家卫生研究院;

关键词：

RETINAL-PIGMENT EPITHELIUM; COMPLEMENT FACTOR-H; STEM-CELLS; DIRECTED DIFFERENTIATION; MESENCHYMAL TRANSITION; OUTER SEGMENTS; ORGANIZATION; PHAGOCYTOSIS; METABOLISM; SECRETION;

D O I：

10.1016/j.exer.2018.10.009

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Human RPE cell lines, especially the ARPE-19 cell line, are widely-used in eye research, as well as general epithelial cell studies. In comparison with primary RPE cells, they offer relative convenience and consistency, but cultures derived from these lines are typically not well differentiated. We describe a simple, rapid method to establish cultures from ARPE-19 cells, with significantly improved epithelial cell morphology and cytoskeletal organization, and RPE-related functions. We identify the presence of nicotinamide, a member of the vitamin B family, as an essential factor in promoting the observed differentiation, indicating the importance of metabolism in RPE cell differentiation.

引用

页码：18 / 24

页数：7

共 36 条

[1] Induction of Differentiation by Pyruvate and DMEM in the Human Retinal Pigment Epithelium Cell Line ARPE-19 [J].

Ahmado, Ahmad ;

Carr, Amanda-Jayne ;

Vugler, Anthony A. ;

Semo, Ma'ayan ;

Gias, Carlos ;

Lawrence, Jean M. ;

Chen, Li Li ;

Chen, Fred K. ;

Turowski, Patric ;

da Cruz, Lyndon ;

Coffey, Peter J. .

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2011, 52 (10) :7148-7159

[2] SELECTIVE PROTEOLYSIS OF ARRESTIN BY CALPAIN - MOLECULAR CHARACTERISTICS AND ITS EFFECT ON RHODOPSIN DEPHOSPHORYLATION [J].

AZARIAN, SM ;

KING, AJ ;

HALLETT, MK ;

WILLIAMS, DS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (41) :24375-24384

[3] Understanding age-related macular degeneration (AMD): Relationships between the photoreceptor/retinal pigment epithelium/Bruch's membrane/choriocapillaris complex [J].

Bhutto, Imran ;

Lutty, Gerard .

MOLECULAR ASPECTS OF MEDICINE, 2012, 33 (04) :295-317

[4] Rapid and Efficient Directed Differentiation of Human Pluripotent Stem Cells Into Retinal Pigmented Epithelium [J].

Buchholz, David E. ;

Pennington, Britney O. ;

Croze, Roxanne H. ;

Hinman, Cassidy R. ;

Coffey, Peter J. ;

Clegg, Dennis O. .

STEM CELLS TRANSLATIONAL MEDICINE, 2013, 2 (05) :384-393

[5] Phenotypic heterogeneity of retinal pigment epithelial cells in vitro and in situ [J].

Burke, JM ;

Skumatz, CMB ;

Irving, PE ;

McKay, BS .

EXPERIMENTAL EYE RESEARCH, 1996, 62 (01) :63-73

[6] The NAD+ Precursor Nicotinamide Riboside Enhances Oxidative Metabolism and Protects against High-Fat Diet-Induced Obesity [J].

Canto, Caries ;

Houtkooper, Riekelt H. ;

Pirinen, Eija ;

Youn, Dou Y. ;

Oosterveer, Maaike H. ;

Cen, Yana ;

Fernandez-Marcos, Pablo J. ;

Yamamoto, Hiroyasu ;

Andreux, Penelope A. ;

Cettour-Rose, Philippe ;

Gademann, Karl ;

Rinsch, Chris ;

Schoonjans, Kristina ;

Sauve, Anthony A. ;

Auwerx, Johan .

CELL METABOLISM, 2012, 15 (06) :838-847

[7] ARPE-19, a human retinal pigment epithelial cell line with differentiated properties [J].

Dunn, KC ;

AotakiKeen, AE ;

Putkey, FR ;

Hjelmeland, LM .

EXPERIMENTAL EYE RESEARCH, 1996, 62 (02) :155-169

[8] Complement factor H polymorphism and age-related macular degeneration [J].

Edwards, AO ;

Ritter, R ;

Abel, KJ ;

Manning, A ;

Panhuysen, C ;

Farrer, LA .

SCIENCE, 2005, 308 (5720) :421-424

[9] Defective phagosome motility and degradation in cell nonautonomous RPE pathogenesis of a dominant macular degeneration [J].

Esteve-Rudd, Julian ;

Hazim, Roni A. ;

Diemer, Tanja ;

Paniagua, Antonio E. ;

Volland, Stefanie ;

Umapathy, Ankita ;

Williams, David S. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (21) :5468-5473

[10]

Fasler-Kan E, 2018, METHODS MOL BIOL, V1745, P305, DOI 10.1007/978-1-4939-7680-5_17

← 1 2 3 4 →